IL-4 Human Matched Antibody Pair/人IL-4匹配抗体对
货号：CHC1283
规格：10 x 96 tests
价格：6895
产品类型：标准品和抗体对
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 15.6 pg/mL |
| 检测范围 | 15.6-1000 pg/mL |
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 30 min |
测试时间 | 24 hr |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 捕获（包被）抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP |
储存温度 | 2-8℃ |
| Protein name | IL-4 |
| Protein aliases | B cell growth factor 1, B-cell stimulatory factor 1, B_cell stimulatory factor 1, Binetrakin, BSF-1, IL-4, interleukin 4 variant 2, Interleukin-4, Lymphocyte stimulatory factor 1, Pitrakinra |
物种（Tested） | 人 |
试剂盒类型 | 匹配抗体对 |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | BCGF-1, BCGF1, BSF-1, BSF1, IL-4, IL4 |
| Gene ID | (Human) 3565 |
| Gene symbol | IL4 |
| UniProt ID | (Human) P05112 |
| The Human IL-4 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-4. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells, and serves as a ligand for interleukin 4 receptor. IL-4 has been found to have the broadest range of actions. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play an important role in mediating the immune regulatory signal of IL-4. The IL-4 gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q. In particular, IL-4 is located in close proximity to IL13 on the chromosome. Further, the IL-4 gene, IL13 and IL5 are found to be coordinated by several long-range regulatory elements over a 120 kilobase range on the chromosome. IL-4 delta 2 is a known splice variant of IL-4.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据： |
IL-4 Human Matched Antibody PairRepresentative standard curve for Human IL-4 ELISA |
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