IL-2 Human Matched Antibody Pair/人IL-2匹配抗体对
货号：CHC1243
规格：10 x 96 tests
价格：6981
产品类型：标准品和抗体对
品牌：Thermo Fisher
物种：人
宿主：其它
抗体亚型：其它
荧光染料：其它
灵敏度 | 31.2 pg/mL |
| 检测范围 | 31.2-1000 pg/mL |
样本类型/体积 | 血清 100 μL 血浆 100 μL 上清液 100 μL |
操作时间 | 1 hr 30 min |
测试时间 | 24 hr |
Homogenous (no wash) | No |
仪器设备 | Colorimetric Microplate Reader 酶标仪 |
规格 | 10 x 96 tests |
组份 | - 捕获（包被）抗体 - 检测抗体 - 标准品 - 链霉亲和素-HRP |
储存温度 | 2-8℃ |
| Protein name | IL-2 |
| Protein aliases | Aldesleukin, IL-2, T-cell growth factor, TCGF, Interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor |
物种（Tested） | 人 |
试剂盒类型 | 匹配抗体对 |
标记（染料） | HRP |
| 检测抗体偶联物 | Biotin |
| Gene aliases | IL-2, IL2, lymphokine, TCGF |
| Gene ID | (Human) 3558 |
| Gene symbol | IL2 |
| UniProt ID | (Human) P60568 |
| The Human IL-2 Antibody Pair Kit comes with pre-matched antibody pairs, standards, and streptavidin-HRP to develop your own enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of human IL-2. Buffer reagents needed to complete the ELISA reaction are sold separate as a Buffer Kit for Antibody Pairs (Catalog No. CNB0011). The Assay Buffer included in this Buffer Kit can be used as a blocking reagent for ELISA plates as well as a diluent for ELISA standards and samples, detection antibody, and HRP conjugate.Principle of the methodELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.Target informationInterleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据： |
IL-2 Human Matched Antibody PairRepresentative standard curve for Human IL-2 ELISA |
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