MLL1 Polyclonal Antibody

货号：A300-374A

规格：100 µL

价格：5699

产品类型：标签抗体

品牌：Thermo Fisher

抗原：Between 2725 and 2775

物种：人

宿主：兔

抗体亚型：IgG

荧光染料：其它

类型:	一抗	同型对照：
浓度：	1 mg/mL	用法：	2-5 µg/mg lysate(IP);1:2,000-1:10,000(WB)

产品详细信息The recommended shelf life for this product is 1 year from date of receipt.Based on 100% sequence identity, this antibody is predicted to react with Mouse 靶标信息KMT2A is a histone methyltransferase that plays an essential role in early development and hematopoiesis. It is a catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac). In the MLL1/MLL complex, KMT2A specifically mediates H3K4me, a tag for epigenetic transcriptional activation. KMT2A has weak methyltransferase activity by itself, and requires other components of the MLL1/MLL complex to obtain full methyltransferase activity. It has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', and has higher activity toward H3 acetylated on 'Lys-9'. Required for transcriptional activation of HOXA9 and promotes PPP1R15A-induced apoptosis.

数据：

MLL1 Antibody (A300-374A) in WBDetection of human MLL1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MLL1 antibody A300-374A (lot A300-374A-5) used for IP at 3 µg per reaction. MLL1 was also immunoprecipitated by rabbit anti-MLL1 antibody A300-375A. For blotting immunoprecipitated MLL1, A300-374A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. MLL1 Antibody (A300-374A) in ChIP ChIP-chip scatter plot of anti-MLL1 (A300-374A) enriched DNA binding sites versus input reference DNA. A. 10 µg of A300-374A was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16: 245-256). immunoprecipitated DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites. B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the anti-MLL1 ChIP, normal rabbit IgG showed little enrichment.

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