| The Human Interferon alpha Multisubtype (Hu IFN-aA, IFN-a2, IFN-aD, IFN-aB2, IFN-aC, IFN-aG, IFN-aH, IFN-aI, IFN-aJ1, IFN-aK, IFN-a1, IFN-a4a, IFN-a4b, and IFN-aWA)人干扰素α多亚型（Hu IFN-aA，IFN-a2，IFN-aD，IFN-aB2，IFN-aC，IFN-aG，IFN-aH，IFN-aI，IFN-aJ1，IFN-aK，IFN-a1 ，IFN-a4a，IFN-a4b和IFN-aWA）ELISA quantitates Hu IFNα subtypes in human cell culture medium. The assay will exclusively recognize both natural and recombinant Hu IFNα subtypes.ELISA定量了人细胞培养基中的IFNα亚型。该检测试剂盒将专门识别天然和重组HuIFNα亚型。Principle of the method 方法原理The Human IFNα subtypes solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.人IFNα亚型固相夹心ELISA（酶联免疫吸附测定）设计用于测量匹配的抗体对之间结合的靶标量。已在提供的微孔板的孔中预包被了靶标特异性抗体。然后将样品，标准液或质控品添加到这些孔中，并与固定的（捕获）抗体结合。通过添加第二（检测）抗体形成三明治，添加底物溶液，该底物溶液与酶-抗体-靶标复合物反应以产生可测量的信号。该信号的强度与原始样本中存在的目标浓度成正比。Rigorous validation 严格验证Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.此ELISA试剂盒的每个制造批次均经过质量测试，其标准包括敏感性，特异性，精密度和批次间一致性。有关验证的更多信息，请参见手册。Target information 靶标信息IFN alpha (Interferon alpha) is a Type I interferon belonging to a family of structurally related, species-specific proteins exclusive to vertebrates. The interaction of IFN-alpha with its receptor component result in the activation of a number of signaling pathways which are mainly involved in innate immune response against viral infection. In humans, the gene for IFN alpha is located on on chromosome 9. The type I interferons consist of 14 different alpha isoforms (subtypes with slightly different specificities), and single beta, omega, epsilon and kappa isoforms. Homologous molecules are found in many species, including rats and mice (and most mammals), and have been identified in birds, reptiles and fish species. IFN alpha is produced primarily by plasmacytoid dendritic cells, is a potent component of the anti-viral innate immune response and modulates adaptive immunity. Diseases associated with the dysfunction of IFN alpha include Hepatitis C and Hepatitis.IFNα（干扰素α）是一种I型干扰素，属于脊椎动物专有的结构相关的物种特异性蛋白家族。 IFN-α与其受体组分的相互作用导致许多信号通路的活化，这些信号通路主要参与针对病毒感染的先天免疫应答。在人类中，IFNα的基因位于9号染色体上。I型干扰素由14种不同的α同工型（特异性稍有不同的亚型）以及单个β，ω，ε和κ同工型组成。同源分子存在于许多物种中，包括大鼠和小鼠（以及大多数哺乳动物），并且已经在鸟类，爬行动物和鱼类中得到鉴定。 IFNα主要由浆细胞样树突状细胞产生，是抗病毒先天免疫应答的有效成分，可调节适应性免疫。与IFNα功能障碍有关的疾病包括丙型肝炎和肝炎。For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.仅供研究使用。 不用于诊断过程。 未经明确授权不得转售。 |
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