CD16 Monoclonal Antibody (B73.1), Functional Grade, eBioscience™/CD16单克隆抗体 (B73.1), Functional Grade, eBioscience™

货号：16-0167-82,16-0167-85,16-0167-38,16-0167-025,16-0167-050

规格：100ug,500ug,5 mg,25 mg,50 mg

价格：2654,4697,24836,74795,124297

产品类型：流式抗体

品牌：eBioscience

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: This B73.1 monoclonal antibody reacts with human CD16, a member of the Fc receptor family also known as Fc gamma RIIIa and Fc gamma RIIIb. This 50-65 kDa receptor is expressed on natural killer cells, neutrophils, monocytes, and macrophages. Crosslinking of CD16 in natural killer cells and monocytes results in activation of the MAPK and PI3K signaling pathways, thus regulating the production of TNF alpha, IL-6, and IL-1 beta.This monoclonal antibody has been shown to block Fc receptor functions.Applications Reported: This B73.1 antibody has been reported for use in functional blocking assays and flow cytometric analysis.Applications Tested: This B73.1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

数据

CD16 Antibody (16-0167-82)Journal of neurovirology 2017 -Fig. 6 Surface expression of key markers of microglia. a Flow cytometry analysis was used to measure the surface expression of CD11b, CD14, CD68, CD16, CD32, CD64, CD163, P2RY12, and TGFbetaR on the representative immortalized cell line hmuglia 1A1. In each experiment, 100,000 cells were resuspended in 1 mL of cold PBS in the presence of 0.5 mug of the antibody or isotype control for 20 min on ice. Appropriate secondary antibodies were used in the absence of fluorophore-conjugated primary antibody, and the cell-antibody complexes were centrifuged and resuspended in PBS. In each FACS profile, the gray distributions represent the proportion of cells bound to the isotype control, whereas the red distributions represent the proportions of cells bound by the target antibody. b Quantification of the abovementioned markers as well as GFAP, CD4, and CCR5 on the surface of primary human astrocytes and immortalized microglia, as indicated

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参考文献：

1.Journal of neurovirology Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system. "Published figure using CD16 monoclonal antibody (Product # 16-0167-82) in Flow Cytometry" 2.mAbs Recombinant H22(scFv) blocks CD64 and prevents the capture of anti-TNF monoclonal antibody. A potential strategy to enhance anti-TNF therapy. "16-0167 was used in Flow cytometry/Cell sorting to demonstrate the specific blocking of CD64 by H22(scFv) could be used as a mechanism for potentiating the effect of anti-TNF antibodies." AuthorsHristodorov D,Mladenov R,Brehm H,Fischer R,Barth S,Thepen T 3.Scientific reports Extraordinarily potent proinflammatory properties of lactoferrin-containing immunocomplexes against human monocytes and macrophages. AuthorsHu L,Hu X,Long K,Gao C,Dong HL,Zhong Q,Gao XM,Gong FY 4.Cancer immunology research Antibody-Dependent Cellular Cytotoxicity Activity of a Novel Anti-PD-L1 Antibody Avelumab (MSB0010718C) on Human Tumor Cells. "16-0167 was used in Neutralization experiments to investigate the effect if avelumab on tumour cells." AuthorsBoyerinas B,Jochems C,Fantini M,Heery CR,Gulley JL,Tsang KY,Schlom J

技术参数

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