| 产品介绍 | | |
| Recombinant human macrophage colony stimulating factor (M-CSF) expressed inE. coli. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified recombinant protein - lyophilized |
Reconstitution | Reconstitute in sterile distilled water to 0.1-0.5 mg/ml. |
Buffer Solution | Phosphate buffered saline |
Preservative Stabilisers | None present |
Carrier Free | Yes |
Activity | The ED50was determined by the dose dependent stimulation of the proliferation of murine M-NSF-60 cells is 1.0 ng/ml, corresponding to a specific activity of 1 x 106units/mg. |
Regulatory | For research purposes only |
Guarantee | Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date. |
| Endotoxin Level | < 0.1 ng/ug |
Purity | >98% by SDS PAGE and HPLC analyses |
| 存储条件 | | |
| Store at -20℃only.Storage in frost-free freezers is not recommended.This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | | |
Functional Assays | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
| 文献 |
| 1. Ralph, P.et al.(1986) Molecular and biological properties of human macrophage growth factor, CSF-1.Cold Spring Harb Symp Quant Biol. 51:679 - 6832. MacDonald, B.R.et al.(1986) Effects of human recombinant CSF-GM and highly purified CSF-1 on the formation of multinucleated cells with osteoclast characteristics in long-term bone marrow culturesJ Bone Miner Res. 1: 227 - 233.3. Ralph, P.et al.(1986) Biological properties and molecular biology of the human macrophage growth factor, CSF-1.Immunobiology. 172: 194 - 204.4. Wong, G.G. (1987) Human CSF-1: molecular cloning and expression of 4-kb cDNA encoding the human urinary protein.Science. 235: 1504-1508 |
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