Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒
货号：K0651,K0652
规格：10 reactions,30 reactions
价格：882,2261
产品类型：核酸标记
品牌：Thermo Fisher
| Thermo Scientific Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein. The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C. Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity. The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template. You can uniformly label any length DNA fragments.Biotin-labeled DNA is detected with theBiotin Chromogenic Detection Kitor conventional biotin-avidin or biotin-streptavidin detection systems.PrincipleRandom decamers are annealed to a denatured template DNA molecule, and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand. |
优点： |
| - Non-radioactivelabeling of DNA- Efficientpriming of labeling reactions with random decamers- High yieldswith Klenow Fragment, exo-: no degradation of a labeled probe during reaction |
数据： |
图1.Dot-blot hybridization with a biotin-labeled probeLambda DNA/HindIII was biotin-labeled with the Biotin DecaLabel DNA Labeling Kit and used as a hybridization probe in a dot-blot of the homologous DNA on SensiBlot Plus Nylon Membrane. The blot was developed with the Biotin Chromogenic Detection Kit. |
组成成分： |
| ▪Klenow Fragment, exo–, 5 u/μl▪Decanucleotide in 5X Reaction Buffer▪Biotin Labeling Mix（1 mM dGTP, 1 mM dATP, 1 mM dCTP, 0.65 mM dTTP, 0.35 mM Biotin-11-dUTP aqueous solution）▪Control Template （λ DNA/HindIII fragments,10 ng/µl）▪Biotin-labeled DNA （50 µl of biotin-labeled DNA (λ DNA/HindIII), 5 ng/µl）▪Water, nuclease-free |
相关产品： |
| ▪DecaLabel DNA Labeling Kit/DNA标记试剂盒（货号#K0622）▪Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒（货号#0651）▪Biotin Chromogenic Detection Kit/生物素显色检测试剂盒（货号#0661） |
技术参数 产品应用 Generation of biotin-labeled DNA probes for a variety of non-radioactive hybridization experiments, including Southern and Northern blots, colony/plaque hybridizations, dot/slot blots, and in situ hybridizations.
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