Biotin Chromogenic Detection Kit/生物素显色检测试剂盒
货号：K0661,K0662
规格：10 reactions,K0662
价格：873,1905
产品类型：核酸标记
品牌：Thermo Fisher
| Thermo Scientific Biotin Chromogenic Detection Kit is a convenient tool for the chromogenic detection of biotinylated nucleic acid probes. The kit is optimized to reproducibly provide high sensitivity with a low background in applications, such as Southern, Northern, dot and slot blotting, and screening of viral plaques and bacterial colonies. Biotinylation of DNA/RNA probes is widely used as a safe and convenient alternative to radioactive labeling. Biotin can be incorporated into nucleic acids using various enzymatic or non-enzymatic methods, including theBiotin DecaLabel DNA Labeling Kit.FeaturesBiotinylated probes are detected with streptavidin coupled to alkaline phosphatase (AP). Streptavidin-AP conjugates bind specifically and irreversibly to the biotin-labeled probes. The probes are then visualized using a chromogenic substrate for alkaline phosphatase BCIP/NBT, which produces a blue-purple precipitate. Visualization does not require X-ray film or other specific equipment (seeFigure 1). |
优点： |
| - High sensitivity of detection—0.03 pg of homologous DNA in dot blot hybridization with biotin-labeled probe- Low backgrounddue to optimized washing and blocking procedures- Non-radioactive detectionvisualization does not require X-ray film or other specific equipment (seeFigure 2)- Fast and convenient—ready-to-use components |
数据： |
图1.人CFU-GM集落 |
组成成分： |
| ▪Streptavidin-AP Conjugate▪50X BCIP/NBT Solution▪Blocking Reagent (powder)▪10X Blocking/Washing Buffer▪10X Detection Buffer |
相关产品： |
| ▪DecaLabel DNA Labeling Kit/DNA标记试剂盒（货号#K0622）▪Biotin DecaLabel DNA Labeling Kit/生物素标记DNA标记试剂盒（货号#0651）▪Biotin Chromogenic Detection Kit/生物素显色检测试剂盒（货号#0661） |
技术参数 产品应用 • Southern, Northern, dot/slot blots, plaque, or colony screening.
Note:This kit is optimized for hybridization procedures using nylon membranes. The detection sensitivity may vary depending on the membranes supplied by different vendors.
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