BseMII (BspCNI) (1 U/µL)/BseMII内切酶
货号：ER1401
规格：100 units
价格：959
产品类型：内切酶
品牌：Thermo Fisher
| Thermo Scientific BseMII (BspCNI) restriction enzyme recognizes CTCAG(10/8)^ sites and cuts best at 55°C in Tango(+SAM) buffer (isoschizomers: BspCNI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Incubation at 37°C results in 30% activity. Requires SAM for activity. Sinefungin can replace SAM in the restriction reaction. In this case, DNA is not methylated, and more than 95% of the ligated BseMII fragments can be recut by this enzyme. For methylation sensitivity, refer to product specifications.Thermo Scientific BseMII（BspCNI）限制酶识别CTCAG（10/8）^位点，并在55°C的Tango（+ SAM）缓冲液（同分异构体：BspCNI）中最佳切割。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：在37°C下温育会产生30％的活性。 需要SAM才能进行活动。 Sinefungin可以在限制性反应中代替SAM。 在这种情况下，DNA不会被甲基化，并且超过95％的连接BseMII片段可以被这种酶切割。 有关甲基化敏感性，请参阅产品说明。 |
| 优点： |
| • Superior quality—stringent quality control and industry leading manufacturing process• Convenient color-coded Five Buffer System• Includes universal Tango buffer for double-digestions• BSA premixed in reaction buffers• Wide selection of restriction endonuclease specificities• 优异的质量—严格的质量控制和业内领先的生产流程• 使用颜色标记的五大便捷缓冲系统• 包含适于双酶切的通用型Tango缓冲液• 反应缓冲液中预先混入BSA• 种类齐全，特异性限制性内切酶的广泛选择 |
| 数据： |
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产品优点 • Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities
• 优异的质量—严格的质量控制和业内领先的生产流程
• 使用颜色标记的五大便捷缓冲系统
• 包含适于双酶切的通用型Tango缓冲液
• 反应缓冲液中预先混入BSA
• 种类齐全，特异性限制性内切酶的广泛选择
产品应用 • Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP
•分子克隆
•限制性位点比对
•基因分型
•Southern印迹
•限制性片段长度多态性（RFLP）
•SNP（单核苷酸多态性）
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