| Thermo Scientific AloI restriction enzyme recognizes ^(7/12-13)GAAC(N)6TCC(12-13/7)^ sites and cuts best at 30°C in R buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:For methylation sensitivity, refer to product specifications. Incubation at 37°C results in 20% activity. AloI produces double-strand cuts on both sides from the interrupted recognition site. Its unique feature is a degenerate cleavage point on the 3 side of the recognition sequence (12 or 13nt away). The presence of SAM in the reaction mixture results in incomplete cleavage with AloI. Greater than 10-fold overdigestion with AloI may result in star activity. AloI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis.Thermo Scientific AloI限制性内切酶可识别^（7 / 12-13）GAAC（N）6TCC（12-13 / 7）^位点，并在30°C下在R种缓冲液中切割得最好。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注：对于甲基化的敏感性，请参阅产品说明书。37℃下温育有20％的活性。AloI在中断的识别位点两侧产生双链切割。 它的独特功能是在识别序列的3端（距离12或13 nt）有一个简并的切割点。 反应混合物中SAM的存在导致AloI裂解不完全。 用AloI进行的超过10倍的过量酶切消化可能会导致星号活性。 AloI可保持与切割的DNA缔合。 这可能会在电泳过程中引起DNA条带移动。 为避免出现非典型的DNA条带，在电泳之前，请使用6X DNA上样染料和SDS溶液进行样品制备或在SDS存在的情况下加热消化的DNA。 |
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