| Thermo Scientific I-SceI homing enzyme recognizes TAGGGATAA^CAGGGTAAT sites and cuts best at 37°C in Tango buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Homing enzymes do not have stringently defined recognition sequences. They can tolerate minor sequence changes, which only partially affect the cleavage reaction. I-SceI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. Assayed using pUC-I-SceI DNA.Thermo Scientific I-SceI homing酶识别TAGGGATAA ^ CAGGGTAAT位点，并在Tango缓冲液中于37°C切割效果最佳。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：Homing酶没有严格定义的识别序列。 它们可以忍受较小的序列改变，这仅部分影响切割反应。 I-SceI可保持与切割的DNA缔合。 这可能会在电泳过程中引起DNA条带移动。 为了避免出现非典型的DNA条带图谱，请在电泳前使用6X DNA上样染料和SDS溶液进行样品制备或在SDS存在的情况下加热消化的DNA。 使用pUC-I-SceI DNA进行分析。 |
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