| Thermo Scientific SfiI restriction enzyme recognizes GGCCNNNN^NGGCC sites and cuts best at 50°C in G buffer. SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:Assayed using pUC19 DNA with insert containing two SfiI sites from Adenovirus-2 DNA. Incubation at 37°C results in 10% activity. SfiI cleavage is impaired by overlapping dcm methylation. To avoid dcm methylation, use a dam-, dcm- strain, such as GM2163. For cleavage with SfiI, at least two copies of its recognition sequence are required. The two sites can be on either the same or different DNA molecules. (L. M. Wertzell et al., J. Mol. Biol., 248, 581-595, 1995.) Therefore, also an oligonucleotide harboring a SfiI recognition site can be supplemented. For methylation sensitivity, refer to product specifications.Thermo Scientific SfiI限制性内切酶可识别GGCCNNNN ^ NGGCC位点，并在50°C的G种缓冲液中最佳切割。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：使用pUC19 DNA和带有两个来自Adenovirus-2 DNA的SfiI位点的插入片段进行分析。 在37°C下孵育可产生10％的活性。 dcm甲基化重叠会削弱SfiI切割。为避免dcm甲基化，请使用dam- dcm-菌株，例如GM2163。 为了用SfiI切割，需要至少其识别序列的两个拷贝。 这两个位点可以在相同或不同的DNA分子上。 （L.M.Wertzell等人，J.Mol.Biol。，248，581-595，1995。）因此，还可以补充具有SfiI识别位点的寡核苷酸。 有关甲基化敏感性，请参阅产品说明。 |
QQ:1749072012