| Thermo Scientific FaqI (BsmFI) restriction enzyme recognizes GGGAC(10/14)^ sites and cuts best at 37°C in Tango (+SAM) buffer (isoschizomers: BslFI, BsmFI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as aFastDigest enzymefor rapid DNA digestion.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Note:FaqI requires only Mg2+ for its activity, but is stimulated by S-adenosylmethionine. 0.05mM S-adenosylmethionine gives more than a 2-fold increase in FaqI activity. Still, complete cleavage of some substrates is difficult to achieve. FaqI concentration is determined by the maximum cleavage level achieved when no change in the fragmentation pattern is observed with further enzyme increase. FaqI may remain associated with the cleaved DNA. This may cause DNA band shifting during electrophoresis. To avoid atypical DNA band patterns, use the 6X DNA Loading Dye & SDS Solution for sample preparation or heat the digested DNA in the presence of SDS prior to electrophoresis. For methylation sensitivity, refer to product specifications.Thermo Scientific FaqI（BsmFI）限制性内切酶可识别GGGAC（10/14）^位点，并在37°C的Tango（+ SAM）缓冲液（同分异构体：BslFI，BsmFI）中最佳切割。有关此酶及其他限制酶的酶活性，双重消化条件和热灭活条件，请参见限制酶的反应条件表格。注意：也可以作为FastDigest酶用于快速DNA消化。Thermo Scientific常规限制性内切核酸酶是大量高质量的限制性内切酶，经过优化可在五种缓冲液系统的一种缓冲液中工作。 此外，通用的Tango缓冲液为双消化提供了便利。 在推荐的缓冲液和反应条件下，所有酶均具有100％的活性。 为确保性能稳定，Thermo Scientific限制性内切酶反应缓冲液包含预混合的BSA，可增强许多酶的稳定性并结合DNA制备物中可能存在的污染物。注意：FaqI的活性仅需要Mg2+，但会受到S-腺苷甲硫氨酸的刺激。 0.05mM S-腺苷甲硫氨酸的FaqI活性提高了2倍以上。但是，仍然难以完全裂解某些底物。 FaqI浓度由在进一步酶增加时未观察到片段化模式变化的最大裂解水平决定。FaqI可以保持与切割的DNA缔合。 这可能会在电泳过程中引起DNA条带移动。 为了避免出现非典型的DNA条带图谱，请在电泳前使用6X DNA上样染料和SDS溶液进行样品制备或在SDS存在的情况下加热消化的DNA。 有关甲基化敏感性，请参阅产品说明。 |
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