| Thermo Scientific PscI (PciI) restriction enzyme recognizes A^CATGT sites and cuts best at 37°C in Tango buffer (isoschizomers: PciI). SeeReaction Conditions for Restriction Enzymesfor a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes.Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.Thermo Scientific PscI (PciI) 限制性内切酶可识别 A^CATGT 位点，于 37°C 下在Tango 缓冲液中的切割效果最佳（同裂酶：PciI）。有关本限制性内切酶及其他限制性内切酶的酶活性、双酶切条件和热灭活的表格，请参见限制性内切酶的反应条件。Thermo Scientific 常规限制性核酸内切酶是高质量限制性内切酶的大量汇集物，经过优化以便在五缓冲液系统的一种缓冲液中发挥作用。此外还提供通用 Tango 缓冲液，便于进行双酶切。所有酶在推荐的缓冲液和反应条件下均表现出 100% 活性。为确保一致的性能，Thermo Scientific 限制性内切酶反应缓冲液包含预混合 BSA，其可增强多种酶的稳定性，并与 DNA 制剂中可能存在的污染物相结合。对 dam 甲基化不敏感、对 dcm 甲基化不敏感、对 CpG 甲基化不敏感. |
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