UTP Solution, Tris buffered
货号： R1471
规格：0.25 mL
价格：796
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
| Thermo Scientific UTP (uridine 5'-triphosphate), Tris buffered is an extremely stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with Tris base. The nucleotide has greater than 99% purity, is free of nuclease activities and functionally tested in in vitro transcription. |
优点： |
| - Greater than 99% purity confirmed by HPLC- Free of trace contaminating nucleotides, endo-, exodeoxyribonuclease and ribonuclease activities- Higher in vitro transcription yields using Tris buffered NTPs in comparison to NTPs, titrated with NaOH- High stability – stable for 3 years at -20°C and after multiple freeze-thaw cycles |
数据： |
图1.Better performance inin vitrotranscription using Tris buffered NTPs in comparison to NTPs, titrated with NaOHRNA transcripts of 100 bases were generated from T7in vitrotranscription reactions using Thermo Scientific NTPs, titrated with NaOH (A) and NTPs, Tris buffered (B). Reactions contained 1 µg of DNA template and were incubated for 2 hours at 37°C. 3 repeats of transcription reaction were performed for each group of NTPs. |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • in vitro transcription
• RNA amplification
• siRNA synthesis
• aRNA synthesis
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