Maxima H Minus First Strand cDNA Synthesis Kit, with dsDNase/cDNA合成试剂盒
货号：K1681,K1682
规格：20 reactions,100 reactions
价格：1535,6134
产品类型：PCR 及DNA聚合酶
品牌：Thermo Fisher
| Thermo Scientific Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase provides a dramatically simplified workflow that combines genomic DNA elimination and cDNA synthesis into one-tube procedure. The kit contains a novel double-strand specific DNase (dsDNase) engineered to remove contaminating genomic DNA from RNA preps in 2 minutes without damage to quality or quantity of RNA. Highly specific dsDNase activity towards double-stranded DNA ensures that single-stranded DNA (such as cDNA and primers) is not cleaved and dsDNase treated RNA can be directly added to reverse transcription.For reverse transcription the kit uses Thermo Scientific Maxima H Minus Reverse Transcriptase (RT), which is an advanced enzyme derived byin vitroevolution of M-MuLV RT. The enzyme features the highest thermostability among the derivatives of M-MuLV RT and lacks RNase H activity. The Maxima H Minus First Strand cDNA Synthesis Kit allows synthesis of long cDNAs up to 20 kb at elevated temperatures (up to 65°C), superseding other systems' abilities to produce full-length cDNA. Due to increased synthesis rates the reaction can be completed in 30 minutes.Additional information about reaction components• Maxima H Minus Enzyme Mix containsMaxima H Minus Reverse TranscriptaseandRiboLock RNase Inhibitor. RiboLock RNase Inhibitor effectively protects RNA templates from degradation by RNases A, B and C at temperatures up to 55°C.•oligo(dT)18andrandom hexamerprimers are supplied with the kit. Random hexamer primers bind non-specifically and are used to synthesize cDNA from all RNAs in a total RNA population. The oligo(dT)18primer selectively anneals to the 3'-end of poly(A) RNA, synthesizing cDNA only from poly(A) tailed mRNA. Gene-specific primers may also be used with the kit to prime synthesis from a specified sequence.•10 mM dNTP Mixis a premixed aqueous solution of dATP, dTTP, dCTP, and dGTP.•Nuclease-free wateris provided for reaction set-up and dilution of sample DNA. The absence of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases has been confirmed by appropriate quality tests. |
优点： |
| - Integrated genomic DNA removal step- Increased reaction temperatures—the first strand of cDNA can be synthesized within the 42 to 65°C temperature range- High yields of full-length first strand cDNA—with RNA templates up to 20 kb- Flexible priming—oligo(dT)18, random hexamer, or gene-specific primers |
组成成分： |
| ▪Maxima H Minus First Strand cDNA Synthesis Kit with dsDNase contains Maxima H Minus Enzyme Mix, dsDNase, 10X dsDNase Buffer, Oligo(dT)18and Random hexamer primers, 5X RT Buffer, dNTP Mix, and nuclease-free water. |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • First Strand cDNA synthesis for RT-PCR• Construction of cDNA libraries• Generation of probes for hybridization• Antisense RNA synthesis
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