F(ab')2-Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568/F(ab')2-山羊抗小鼠IgG (H+L)交叉吸附二抗，Alexa Fluor 568

货号：A-11019

规格：500 µg

价格：2787

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 568

抗体类型:	荧光二抗	同型对照：
浓度：	2 mg/mL	用法：	2 µg/mL (ICC)；2 µg/mL(IF)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11019) in IFImmunofluorescence analysis of F (ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 568 conjugate (Product # A-11019) was performed using HeLa cells stained with alpha Tubulin (236-10501) Mouse Monoclonal Antibody (Product # A11126). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, blocked with 1% BSA for 1 hour and labeled with mouse primary antibody (1:250 dilution) for 3 hours at room temperature. F (ab')2-Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor 568 conjugate was used at concentration of 2 µg/mL in phosphate buffered saline containing 0.2 % BSA for 45 minutes at room temperature, for detection of alpha Tubulin in the cytoplasm (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI in SlowFade® Gold Antifade Mountant (Product # S36938). F-actin was stained with Alexa Fluor® 488 Phalloidin (Product # A12379, 1:300) (Panel c: red). Panel d represents the composite image. No nonspecific staining was observed with the secondary antibody alone (panel f), or with an isotype control (panel e). The images were captured at 60X magnification.

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参考文献：

1. Differentiation; research in biological diversityRapid and simple method for in vivo ex utero development of mouse embryo explants.2. Scientific reportsMammalian TRAPPIII Complex positively modulates the recruitment of Sec13/31 onto COPII vesicles.3. Translational psychiatryReversal of neurobehavioral social deficits in dystrophic mice using inhibitors of phosphodiesterases PDE5A and PDE9A.4. Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility SocietyFall in density, but not number of myenteric neurons and circular muscle nerve fibres in guinea-pig colon with ageing.5. Proceedings of the National Academy of Sciences of the United States of AmericaIntracellular mechanisms of molecular recognition and sorting for transport of large extracellular matrix molecules.

技术参数

产品应用 ICC;IF;IHC;IHC(P);Misc

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