| 产品介绍 | | |
| Mouse anti Human Tenascin antibody, clone T2H5recognizes tenascin in both normal and hyperproliferative (also neoplastic) tissues.Tenascin has been implicated in the guidance of migrating neurons and axons during development and neuronal regeneration. It promotes cortical neuron outgrowths and is an important component of the wound matrix and scar formation. |
| 产品详情 | | |
Target Species | Human |
Product Form | Purified IgG - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. |
Buffer Solution | TRIS buffered saline |
Preservative Stabilisers | <0.1% Sodium Azide (NaN3) |
Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
Immunogen | Purified protein from human mammary tumour. |
Regulatory | For research purposes only |
Guarantee | 12 months from date of despatch |
Fusion Partners | Spleen cells from immunized Balb/c mice were fused with cells of the Sp2/0-Ag14 mouse myeloma cell line. |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. |
| 应用 | | |
Application Name | Verified | Min Dilution | Max Dilution |
ELISA | √ | 1/1000 | 1/2000 |
Immunohistology - Frozen | √ | | |
| Immunohistology - Paraffin1 | √ | | |
| Immunoprecipitation | √ | | |
Western Blotting | √ | | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.1Staining may be enhanced using trypsin digestion or heat mediated antigen retrieval using sodium citrate buffer pH 6.0.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.ELISAThis product is suitable for use in direct ELISA applications,8640-0502may be used as a standard.Histology Positive Control TissueHuman tonsil. |
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| 文献 |
| Source Reference1. Schalkwijk, J.et al.(1991) Tenascin expression in human dermis is related to epidermal proliferation.Am J Pathol. 139 (5): 1143-50.References for Tenascin antibody2. Verstraeten, A.A.et al.(1992) Tenascin expression in basal cell carcinoma.Br J Dermatol. 127 (6): 571-4.3. Le Poole, I.C.et al.(1997) Tenascin is overexpressed in vitiligo lesional skin and inhibits melanocyte adhesion.Br J Dermatol. 137 (2): 171-8.4. Milz, S.et al.(2007) An immunohistochemical study of the triangular fibrocartilage complex of the wrist: regional variations in cartilage phenotype.J Anat. 211: 1-7.5. Päiväniemi, O,et al.(2006) Tenascin is expressed in post-transplant obliterative bronchiolitis.Transplant Proc. 38: 2694-6.6. Nakamura, Y.et al.(2004) Ets-1 regulates TNF-alpha-induced matrix metalloproteinase-9 and tenascin expression in primary bronchial fibroblasts.J Immunol. 172: 1945-52.7. Milz, S.et al.(2002) Expression of a wide range of extracellular matrix molecules in the tendon and trochlea of the human superior oblique muscle.Invest Ophthalmol Vis Sci. 43: 1330-4. |
QQ:1749072012