WELQut Protease (5 U/µL)

货号：EO0861

规格：500 units

价格：1499

产品类型：酶及抑制剂

品牌：Thermo Fisher

描述Thermo Scientific WELQut Protease is highly specific, recombinant serine protease ofStaphylococcus aureus. It recognizes and precisely cleaves recombinant proteins containing an engineered recognition sequence† W- E- L- Q↓X (Trp, Glu, Leu, Gln, X can be any amino acid). The protease cleaves outside the recognition sequence without leaving additional amino acids bound to the target protein.The WELQut Protease is active in a broad temperature (4 to 30°C) and pH (pH 6.5 to 9.0) range and does not require specific buffers.In addition, this new protease has several procedural advantages - it is ideal for on-column proteolysis reactions and can be easily removed from reaction mixtures using its built-in His-tag.Highlights• Cleaves outside WELQ recognition sequence, without leaving additional amino acids bound to the target protein• Highly specific to cognate recognition site, does not generate non-specific product bands, even after long incubation and using excess of protease• Easy to remove from the reaction mixture using built-in His-tag• Ideal for on-column proteolysis reactionsApplications• Removal of N-terminal fusion tags from recombinant protein preparations.Footnote† This cleavage sequence is present in expression vector pLATE52 included into theThermo Scientific aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WELQut) (#K1281)available from Thermo Scientific.Highly specific proteolysis of target protein performed by WELQut vs. competitor EnterokinaseTarget protein (Klenow Fragment (exo-), containing WELQut or Enterokinase recognition sequence) was treated with WELQut Protease and Enterokinase according to the recommendations provided by the suppliers. Various enzyme/substrate amount ratios were tested and reaction products were analyzed in SDS-PAGE.M - PageRuler Prestained Protein Ladder (Thermo Scientific, #SM0671).K - Control-uncut target protein.1-6 various WELQut/target protein amount ratios, U/µg.1 - 1:100, 2 - 1:50, 3 - 1:40, 4 - 1:20, 5 - 1:10, 6 - 1:5.7-11 various Enterokinase/target protein amount ratios, µg/µg.7 - 1:100, 8 - 1:40, 9 - 1:20, 10 - 1:10, 11 - 1:5.

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