GeneJET Plasmid Maxiprep Kit/GeneJET质粒DNA大量提取试剂盒
货号：K0491,K0492
规格：10 preps,25 preps
价格：1603,3581
产品类型：DNA提取
品牌：Thermo Fisher
| Thermo Scientific GeneJET Plasmid Maxiprep Kit, sufficient for 25 preps, utilizes silica-based membrane technology in the form of convenient spin column. Each prep recovers up to 750 µg of high copy plasmid DNA that can be used in a wide variety of molecular biology procedures, such as restriction endonuclease digestion, PCR, cloning, transformation, automated sequencing,in vitrotranscription, and transfection of robust cell lines. |
优点： |
| -Efficient—high yields up to 750 µg of high quality plasmid DNA from 250 mL of bacterial culture-High concentration of DNA purified—> 400 ng/ µL-Convenient—spin column format, centrifuge or vacuum protocols-Fast—procedure takes 60 minutes, considerably faster than using gravity flow columns |
数据： |
图1.High yields of plasmid purified with GeneJET Maxiprep KitPlasmid DNA was purified from E. coli cells using the GeneJET Plasmid Maxiprep Kit and the kits of various vendors using recommended culture volumes and purification protocols.图2.Efficient transfection of robust cell lines with plasmid DNA purified using GeneJET Maxiprep Kit- Plasmid DNA, purified from E. coli cells using the GeneJET Plasmid Maxiprep Kit, gives transfection efficiencies comparable to chromatographically purified, endotoxin-free plasmid. Oneμg of the high-copy plasmid transfected into various cell lines using TurboFect in vitro transfection reagent.
- CHR: endotoxin-free plasmid.
- GeneJET: plasmid purified using the GeneJET Plasmid Maxiprep Kit.
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组成成分： |
| ▪Resuspension Solution▪Lysis Solution▪Neutralization Solution▪Endotoxin Binding Reagent▪Wash Solution I (concentrated)▪Wash Solution II (concentrated)▪RNase A Solution▪Elution Buffer (10 mM Tris-HCl, pH 8.5)▪ GeneJET Maxi Purification Columns pre-assembled with
collection tubes (50 mL)▪Collection tubes (50 mL) |
相关产品： |
| ▪DNA 片段标准品▪DNA ladder▪DNA 聚合酶▪PCR▪DNA纯化▪RNA纯化 |
技术参数 产品应用 • Isolated plasmid DNA is suitable for all conventional molecular biology procedures, including:
• Transfection of robust cell lines
• FastDigest restriction enzyme digestion
• Automated fluorescent sequencing
• PCR
• Cloning
• Transformation
• In vitro transcription
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