X-Gal

货号：R0402,R0404

规格：2 x 1 g,1 g

价格：2083,1142

产品类型：分子生物学

品牌：Thermo Fisher

描述Thermo Scientific X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside) is an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal into colorless galactose and 4-chloro-3-brom-indigo, forming an intense blue precipitate. Induction of thelacZgene withIPTGleads to the hydrolysis of X-Gal and to the development of blue colonies (seeSupporting data).Applications• Blue/white colony screening to distinguish recombinant (white) from non-recombinant (blue) colonies (seeReference 1)• Visualization of beta-galactosidase reporter gene expression in transfected eukaryotic cells• Detection of beta-galactosidase activity in immunological and histochemical proceduresNote• Preparation of a 20 mg/mL stock solution in dimethylformamide (DMF) or dimethylsulfoxide (DMSO) is recommended for X-Gal. DMF dissolves some plastic materials. The direct addition of DMF containing solution to plastic Petri dishes should be avoided.• DMF is toxic. Allow X-Gal solution containing dimethylformamide to completely dry before plating bacteria on agar plates.Induction of the lacZ gene with IPTG

技术参数

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