CD28 Monoclonal Antibody (JJ319), Functional Grade, eBioscience™/CD28 单抗 (JJ319), 功能抗体, eBioscience™

货号：16-0280-81,16-0280-85,16-0280-38,16-0280-025,16-0280-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1156,3735,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：大鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：JJ319

荧光染料：其它

_ueditor_page_break_tag_产品详细信息Description: The JJ319 monoclonal antibody reacts with the rat CD28 molecule, expressed by thymocytes, mature T cells, and a subset of NK cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent costimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells. No reactivity of JJ319 is observed on mouse T cells.Applications Reported: The JJ319 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro T cell costimulation.Applications Tested: This JJ319 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.仅用于科研。不用于诊断过程。未经明确授权不得转售。

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