CD3 Monoclonal Antibody (eBioG4.18 (G4.18)), Functional Grade, eBioscience™/CD3 单抗 (eBioG4.18 (G4.18)), 功能抗体, eBioscience™

货号：16-0030-85,16-0030-38,16-0030-025,16-0030-050

规格：500 µg,5 mg,25mg,50mg

价格：3735,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD3

物种：大鼠

宿主：小鼠

抗体亚型：其它

克隆号：eBioG4.18 (G4.18)

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgG3, kappa
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activateT cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.Applications Reported: This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis, immunoblotting (WB), and immunohistochemical staining. The eBioG4.18 antibody has also been used in functional assays.Applications Tested: This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD3 Antibody (16-0030-85) in FlowStaining of rat splenocytes with 0.25 µg of Mouse IgG3 Isotype Control Purified (Product # 14-4742-82) (open histogram) or 0.25 µg of Anti-Rat CD3 Functional Grade Purified (filled histogram) followed by Anti-Mouse IgG Biotin (Product # 13-4013-85)and Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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参考文献：

流式1.PloS oneFringe controls naïve CD4(+)T cells differentiation through modulating notch signaling in asthmatic rat models."16-0030 was used in Flow cytometry/Cell sorting to assess the expression levels of three Fringe homologs in naive CD4(+)T cells in asthmatic rats."AuthorsGu W,Xu W,Ding T,Guo X2.Frontiers in behavioral neuroscienceInterplay between Depressive-Like Behavior and the Immune System in an Animal Model of Prenatal Dexamethasone Administration."16-0030 was used in Flow cytometry/Cell sorting to investigate the long-term impact of prenatal dexamethasone administration in depressive-like behavior and the immune system."AuthorsRoque S,Oliveira TG,Nobrega C,Barreira-Silva P,Nunes-Alves C,Sousa N,Palha JA,Correia-Neves M功能检测3.American journal of translational researchSevere traumatic hemorrhagic shock induces compromised immune barrier function of the mesenteric lymph node leading to an increase in intestinal bacterial translocation."16-0030 was used in Functional assays to explore the mechanism underlying the process of bacterial translocation to extraintestinal tissues under severe shock."AuthorsZhang J,Zhang Y,Xu T,Pan SJ,Nie G,Miao XY,Qiu JY,Yu WQ,Zhang SY,Liang TB4.Mediators of inflammationDeficits in endogenous adenosine formation by ecto-5'-nucleotidase/CD73 impair neuromuscular transmission and immune competence in experimental autoimmune myasthenia gravis."16-0030 was used in Functional assays to investigate the role of ecto-5'-nucleotidase/CD73 in a rat model of experimental autoimmune myasthenia gravis."AuthorsOliveira L,Correia A,Cristina Costa A,Guerra-Gomes S,Ferreirinha F,Magalhães-Cardoso MT,Vilanova M,Correia-de-Sá PIHC5.PloS oneMolecular pathology of murine ureteritis causing obstructive uropathy with hydronephrosis."Published figure using CD3 monoclonal antibody (Product # 16-0030-85) in Immunohistochemistry"

技术参数

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