North2South Biotin Random Prime DNA Labeling Kit

货号：17075

规格：10 reactions

价格：7380

产品类型：核酸标记

品牌：Thermo Fisher

Thermo Scientific North2South Random Prime DNA Biotinylation Kit uses random heptanucleotides, Klenow fragment and biotin-nucleotides to produce biotinylated DNA templates for DNA hybridization and detection methods.Features of the North2South Random Prime DNA Biotinylation Kit:• Works with as little as 100ng starting DNA template• Exonuclease-free Klenow fragment in the DNA labeling kit produces higher yields• Each reaction yields probe sufficient for approximately three (10 x 10 cm) blotsThis random prime DNA labeling kit is based on the procedure of Feinberg and Vogelstein (Ref.4,5). Random heptanucleotides containing all possible sequences are annealed to a denatured DNA template. These act as primers for complementary strand synthesis by DNA Polymerase (Klenow fragment, 3'-5' exo-). Biotinylated dNTPs in the reaction mix are incorporated into the newly synthesized DNA. This protocol yields biotin-labeled DNA of high activity for use as probes in hybridization experiments such as Southern and Northern hybridizations. The resulting hybrids can be detected with streptavidin-horseradish peroxidase (HRP) and a chemiluminescent substrate kit such asNorth2South Chemiluminescent Hybridization and Detection Kit.

技术参数

组成成分 Sufficient For: 10 biotin-labeling reactions (each with 100 ng DNA)• Heptanucleotide Mix (5X), 100 µL• Deoxynucleotide Mix (5X), 80 µL• Reaction Buffer (10X), 50 µL• DNA Polymerase Klenow Fragment (5U/µL), 10 µL• Control DNA (250 ng/µL), 5 µL• Ammonium Acetate (5M), 1 mL• EDTA (0.5M, pH 8.0), 1 mL• Nuclease-free Water, 1 mL• Biotin-11-dUTP, 20 µL

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