CD152 (CTLA-4) Monoclonal Antibody (9H10), Functional Grade, eBioscience™/CD152 (CTLA-4) 单抗 (9H10), 功能抗体, eBioscience™

货号：16-1521-82,16-1521-85,16-1521-38,16-1521-025,16-1521-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1865,3274,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD152

物种：小鼠

宿主：小鼠

抗体亚型：其它

克隆号： 9H10

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Syrian Hamster IgG Isotype Control, Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The 9H10 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.Applications Reported: The 9H10 antibody has been reported for use in flow cytometric analysis. It has also been reported for use in in vitro functional studies.Applications Tested: The 9H10 antibody has been tested by flow cytometric analysis of resting and Con A-stimulated mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

CD152 (CTLA-4) Antibody (16-1521-82)Oncology letters 2018 -Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 16-1521-82)

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参考文献：

流式1.Frontiers in cellular neuroscienceTargeting immune co-stimulatory effects of PD-L1 and PD-L2 might represent an effective therapeutic strategy in stroke."16-1521 was used in Flow cytometry/Cell sorting to investigate PD-L1 and PD-L2 expressing cell types that affect T-cell activation post stroke, and how these could be targeted therapeutically."AuthorsBodhankar S,Chen Y,Lapato A,Vandenbark AA,Murphy SJ,Offner H2.PloS oneRadiotherapy-induced anti-tumor immunity contributes to the therapeutic efficacy of irradiation and can be augmented by CTLA-4 blockade in a mouse model."16-1521 was used in Flow cytometry/Cell sorting to indicate that tumor-specific immune responses play an important role in the therapeutic efficacy of irradiation."AuthorsYoshimoto Y,Suzuki Y,Mimura K,Ando K,Oike T,Sato H,Okonogi N,Maruyama T,Izawa S,Noda SE,Fujii H,Kono K,Nakano T功能检测3.OncoimmunologyCD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b+T cell lymphoma.4.Oncology lettersPotential function of CTLA-4 in the tumourigenic capacity of melanoma stem cells."16-1521 was used in Functional assays to demonstrate that melanoma cell-intrinsic cytotoxic T lymphocyte antigen-4 induces tumour cell proliferation in vitro and suppresses tumour cell apoptosis."中和5OncoimmunologyCD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b+T cell lymphoma.

技术参数

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