Quant-iT OliGreen ssDNA Assay Kit
货号：O11492
规格：1 kit
价格：7527
产品类型：核酸标记
品牌：Thermo Fisher
| OurQuant-iT OliGreen ssDNA Assay Kitcontains an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. In addition to the dye, the kit also includes buffer and an oligonucleotide standard. The OliGreen® reagent is also available as a stand alone product ([PRODUCT O7582]). |
| 数据 |
Linear quantitation of a synthetic 24-mer from 0.1 to 1000 ng⁄mL using the OliGreen® ssDNA quantitation reagent.Linear quantitation of a synthetic 24-mer (an M13 sequencing primer) from 0.1 to 1000 ng⁄mL using the OliGreen® ssDNA quantitation reagent (O7582, O11492). Samples in 10 mm × 10 mm cuvettes were excited at 480 nm. The fluorescence emission intensity was measured at 520 nm using a spectrofluorometer and plotted as a function of oligonucleotide concentration. The inset shows an enlargement of the results obtained with oligonucleotide concentrations between zero and 2.0 ng⁄mL. |
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