CD272 (BTLA) Monoclonal Antibody (6F7), Functional Grade, eBioscience™/CD272 (BTLA) 单克隆 (6F7), 功能抗体, eBioscience™

货号： 16-5950-82,16-5950-025,16-5950-050

规格：100 µg,25 mg,50 mg

价格：3496,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD272

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：6F7

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）

产品详细信息Description: The BTLA antibody reacts with mouse BTLA, B and T lymphocyte attenuator. BTLA is expressed by peripheral lymphocytes, splenic macrophages, developing B cells in the bone marrow and developing T cells in the thymus and mature, but not immature bone marrow-derived dendritic cells. BTLA has been implicated as a negative regulator of the activation and/or function of various hemopoietic cell types. It is reported that BTLA binds to B7-H4, but further studies are needed to confirm this interaction.Note: The anti-mouse BTLA monoclonal antibody 6F7 is reported to stain CD4^+ and CD8^+ single-positive (SP) thymocytes (Hurchla et al). However, other anti-mouse BTLA clones generated simultaneously with 6F7 (8F4, 3F9.D12, 6G3 and 6H6) do not stain SP thymocytes. It is not understood why there is a discrepancy in thymocyte staining however clones 8F4, 3F9.D12, 6G3 and 6H6 stain similar populations to 6F7 in splenocytes and bone marrow cells.Applications Reported: This 6F7 antibody has been reported for use in flow cytometric analysis and has also been reported to block binding of BTLA ligands.Applications Tested: This 6F7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息BTLA or B and T-lymphocyte attenuator is a member of the co-inhibitory receptors of the CD28 superfamily. BTLA is a lymphocyte inhibitory receptor which inhibits lymphocytes during immunes responses. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells, but not Th2 cells. BTLA is a unique co-receptor that interacts with the tumor necrosis factor receptor superfamily member herpesvirus entry mediator (HVEM). This interation is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.仅用于科研。不用于诊断过程。

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参考文献：

流式1.Journal of immunology (Baltimore, Md. : 1950)B and T lymphocyte attenuator suppresses IL-21 production from follicular Th cells and subsequent humoral immune responses."16-5950 was used in Flow cytometry/Cell sorting to suggest that B and T lymphocyte attenuator signaling suppresses IL-21 production by Tfh cells and subsequent Tfh cell-mediated IgG responses."AuthorsKashiwakuma D,Suto A,Hiramatsu Y,Ikeda K,Takatori H,Suzuki K,Kagami S,Hirose K,Watanabe N,Iwamoto I,Nakajima H2.PLoS pathogensHTLV-1 bZIP Factor Enhances T-Cell Proliferation by Impeding the Suppressive Signaling of Co-inhibitory Receptors."16-5950 was used in Flow cytometry/Cell sorting to study how HTLV-1 bZIP factor influences the expression and function of multiple co-inhibitory receptors to induce proliferation of infected T-cells."AuthorsKinosada H,Yasunaga JI,Shimura K,Miyazato P,Onishi C,Iyoda T,Inaba K,Matsuoka M

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