CD278 (ICOS) Monoclonal Antibody (ISA-3), Functional Grade, eBioscience™/CD278 (ICOS)单克隆 (ISA-3), 功能抗体, eBioscience™

货号：16-9948-82,16-9948-025,16-9948-050

规格：100 µg,25 mg,50 mg

价格：3624,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD278

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：ISA-3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The ISA-3 monoclonal antibody reacts with human ICOS (Inducible COStimulatory molecule), also known as H4, CRP-1 and AILIM. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. Human ICOS has a relative molecular mass of 55-60 kDa, composed of 27 kDa and 29 kDa chains. Human ICOS on activated T cells has potent costimulatory activity for T cell activation and is required for humoral immune responses, in particular for memory B cell and plasma cell generation. ICOS binds to its ligand, B7h/B7RP-1 expressed on activated APCs (antigen presenting cells) and on a number of inflamed peripheral tissues. Plate-bound ISA-3 is costimulatory for T cells and induces production of IL-4, IL-5, IL-10 and other cytokines, but not IL-2. ISA-3 has the same reactivity pattern and characteristics as F44. ISA-3 was generated against the human ICOS antigen. C398.4A, anti-mouse ICOS/H4 (catalog # 14-9949-82), was shown to cross-react with human ICOS but binds to an epitope different from ISA-3. C398.4A stains activated cells brighter than ISA-3; however, it also exhibits higher staining of non-activated human peripheral blood or isolated PBMC. To achieve the brightest staining of ICOS on activated human T cells, please use 13-9948-82 or 12-9948-42 rather than 11-9948-42.Applications Reported: The ISA-3 antibody has been reported for use in flow cytometric analysis. It has also been reported in in vitro functional assays.Applications Tested: The ISA-3 antibody has been tested by flow cytometric analysis of unstimulated and PHA-activated (2 days) human blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.仅用于科研。不用于诊断过程。

数据

CD278 (ICOS) Antibody (16-9948-82) in FlowStaining of Anti-Human CD3/CD28-stimulated (Product # 16-0039-81andProduct # 16-0289-81) with Anti-Human CD278 (ICOS) FITC (left) and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

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参考文献：

流式1.OncoimmunologyPolyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS."16-9948 was used in Flow cytometry/Cell sorting to investigate polyfunctionality of CD8(+) T cells relating to AKT activation."2.JCI insightCXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9948-82) in Flow Cytometry"3.Molecular medicine reportsCD28 family of receptors on Tcells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9948-82) in Flow Cytometry"功能检测4.OncoimmunologyTumor-infiltrating plasmacytoid dendritic cells promote immunosuppression by Tr1 cells in human liver tumors."16-9948 was used in Functional assays to investigate the role of T regulatory cells in human solid tumours."5.Journal of immunology (Baltimore, Md. : 1950)Inducible costimulator: a modulator of IFN-gamma production in human tuberculosis.AuthorsQuiroga MF,Pasquinelli V,Martínez GJ,Jurado JO,Zorrilla LC,Musella RM,Abbate E,Sieling PA,García VE

技术参数

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