CD278 (ICOS) Monoclonal Antibody (7E.17G9), Functional Grade, eBioscience™/CD278 (ICOS) 单克隆 (7E.17G9), 功能抗体, eBioscience™

货号：16-9942-81,16-9942-85

规格：50 µg,500 µg

价格：1328,3604

产品类型：流式抗体

品牌：eBioscience

抗原：CD278

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：7E.17G9

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/test（Flow）

产品详细信息Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.Applications Reported: 7E.17G9 has been reported for use in flow cytometric analysis and for inhibition of ligand binding in functional assays.Applications Tested: The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and Con A activated (3 day) mouse splenocytes and can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD278 belongs to the CD28 and CTLA-4 cell-surface receptor family and is highly expressed in activated T-cells. It is essential both for efficient interaction between T and B-cells and for normal antibody responses to T-cell dependent antigens. It enhances all basic T-cell responses to a foreign antigen, namely proliferation, secretion of lymphokines, up-regulation of molecules that mediate cell-cell interaction, and effective help for antibody secretion by B-cells.仅用于科研。不用于诊断过程。

数据

CD278 (ICOS) Antibody (16-9942-81) in FlowStaining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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参考文献：

流式1.Nature communicationsRoquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9942-81) in Flow Cytometry"2.Nature communicationsExtrafollicular CD4+T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9942-81) in Flow Cytometry"3.Nature communicationsTargeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment."Published figure using CD278 (ICOS) monoclonal antibody (Product # 16-9942-81) in Flow Cytometry"4.Genes and developmentThe E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis."16-9942 was used in Flow cytometry/Cell sorting to show that Id2 and Id3 suppress the development and expansion of innate variant follicular helper T cells."AuthorsMiyazaki M,Miyazaki K,Chen S,Chandra V,Wagatsuma K,Agata Y,Rodewald HR,Saito R,Chang AN,Varki N,Kawamoto H,Murre C功能检测5.Journal of immunology (Baltimore, Md. : 1950)The number of responding CD4 T cells and the dose of antigen conjointly determine the TH1/TH2 phenotype by modulating B7/CD28 interactions."16-9942 was used in Functional assays to show that a greater number of Ag-dependent CD4 T cell interactions are required to generate Th2 than Th1 cells."AuthorsRudulier CD,McKinstry KK,Al-Yassin GA,Kroeger DR,Bretscher PA

技术参数

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