| The ViewRNA Cell Plus Assay is a novel assay that combines immunocytochemistry with ViewRNA technology, a proprietary fluorescent in situ hybridization and sequential branched-DNA amplification technique, to visualize both RNA with single-molecule sensitivity and protein in individual cells. This assay enables simultaneous detection of up to three RNA targets in combination with immunophenotyping for cell surface or intracellular proteins using both indirect and direct immunocytochemistry to allow for detailed characterization of specific cell subpopulations. For simultaneous detection of a fourth RNA target, the ViewRNA ISH Cell 740 Module (Cat. No. QVC0200) can be combined with this kit. It allows analysis of an addtional target in the 740 channel (AlexaFluor 750).This ViewRNA Cell Plus Assay kit contains all the reagents needed to conduct the assay. Target Probe sets for genes of interest and antibodies are sold separately.Reactivity/speciesProbes can be designed for any species. These are the species most frequently used/tested: Armenian hamster, baboon, bovine, canine, cat, chicken, chimpanzee, cow, cynomolgus monkey, donkey, goat, golden Syrian hamster, guinea pig, hamster, horse, human, macaque, monkey, mouse, non-human primate, olive baboon, pig, pigtailed macaque, rabbit, rat, rhesus monkey, sheep |
Simultaneous detection of RNAs and proteinMultiplexed, simultaneous detection of POLR2A and ERBB2 mRNA with Golgin97 protein in HeLa cells using the ViewRNA Cell Plus Assay Kit. Golgin97 protein was detected using an eFluor 660 labeled Golgin97 antibody (red). POLR2A and ERBB2 mRNAs were detected using Type 1 (yellow, ERBB2) and Type 4 (green, POLR2A) fluorescent ViewRNA probes. Nuclei were stained with DAPI. Simultaneous detection of RNAs and proteinMultiplexed, simultaneous detection of alpha-tubulin and HPRT1 mRNA with beta-actin protein in HeLa cells using the ViewRNA Cell Plus Assay Kit. Beta-actin protein was detected using an Alexa Fluor 488 labeled beta-actin antibody (green). Alpha-tubulin and HPRT1 mRNAs were detected using Type 1 (yellow, tubulin) and Type 6 (red, HPRT1) fluorescent ViewRNA probes. Nuclei were stained with DAPI. Simultaneous detection of mRNA, microRNA and proteinMultiplexed, simultaneous detection of Let-7A microRNA and GAPDH mRNA with Ki67 protein in HeLa cells using the ViewRNA Cell Plus Assay Kit. Ki67 protein was detected using an eFluor 660 labeled Ki67 antibody (red). Let-7A microRNA and GAPDH mRNA were detected using Type 1 (yellow, Let-7A) and Type 4 (green, GAPDH) fluorescent ViewRNA probes. Nuclei were stained with DAPI. |
QQ:1749072012