North2South Chemiluminescent Hybridization and Detection Kit
货号：17097
规格：10 blots
价格：9498
产品类型：核酸标记
品牌：Thermo Fisher
| Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera. |
| 特点 |
| • Sensitivity equal to or greater than32P• Much faster than DIG detection systems• Amenable to nearly any DNA or RNA hybridization method• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background |
技术参数 组成成分 Sufficient For: 1000 cm2 of membrane (10 mini-gel blots)• Stabilized Streptavidin-HRP, 1.5 mL• Luminol/Enhancer Solution, 80 mL• Stable Peroxide Solution, 80 mL• Hybridization Buffer, 125 mL• Hybridization Stringency Wash Buffer (2X), 375 mL• Blocking Buffer, 500 mL• Wash Buffer (4X), 500 mL• Substrate Equilibration Buffer, 500 mL
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