IL-12 p70 Monoclonal Antibody (20C2), Functional Grade, eBioscience™/IL-12 p70 单克隆 (20C2), 功能抗体, eBioscience™
货号： 16-8126-85,16-8126-38,16-8126-025,16-8126-050
规格：500 µg,5 mg,25 mg,50 mg
价格：3496,24089,68175,113625
产品类型：流式抗体
品牌：eBioscience
抗原：IL-12 p70
物种：人
宿主：大鼠
抗体亚型：其它
克隆号：20C2
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | IgG1 |
浓度： | 1 mg/mL | 用法： | Assay-Dependent（FN）;Assay-Dependent（ELISA）;Assay-Dependent（WB） |
产品详细信息Description: The monoclonal antibody 20C2 reacts with heterodimeric form of IL-12 called p70 (sometimes called p75). The two covalently linked subunits that comprise IL-12 are p35 and p40 with a combined relative mass of 75 kDa protein. IL-12 is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-12 can induced IFN gamma production and plays a key role in the development of Th responses. This 20C2 antibody has been reported to have neutralizing activity of the intactIL-12p70 complex (but not the individual subunits).Applications Reported: This 20C2 antibody has been reported for use in western blotting, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, and ELISA. It has also been reported in blocking IL-12 receptor binding and biologic activity in fucntional assays.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-12 (IL-12) is a heterodimeric 70 kDa cytokine composed of two covalently linked, glycosylated chains with molecular weights of 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation,and cytotoxicity mediated by natural killer cells and T cells. Studies have established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation. Recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. IL-12 p70 is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity. Human and mouse IL-12 p40 share about 70% amino acid sequence homology.仅用于科研。不用于诊断过程。未经明确授权不得转售。
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参考文献： |
| ELISA1.OncoimmunologyHematopoietic stem cell-derived myeloid and plasmacytoid DC-based vaccines are highly potent inducers of tumor-reactive T cell and NK cell responsesex vivo."16-8126 was used in an ELISA assay to indicate that hematopoietic stem and progenitor cells-derived dendritic cell vaccines may possess superior potential to boost antitumour immunity post allogeneic stem cell transplantation."AuthorsThordardottir S,Schaap N,Louer E,Kester MG,Falkenburg JH,Jansen J,Radstake TR,Hobo W,Dolstra H |
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