IL-2 Monoclonal Antibody (JES6-1A12), Functional Grade, eBioscience™/IL-2 单克隆 (JES6-1A12), 功能抗体, eBioscience™

货号：16-7022-81,16-7022-85,16-7022-38,16-7022-95,16-7022-025,16-7022-050

规格：50 µg,500 µg,5 mg,10 mg,25 mg,50 mg

价格：1044,3274,24089,29225,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-2

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：JES6-1A12

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.250 µg/mL（ELISA）

产品详细信息Description: The JES6-1A12 antibody reacts with mouse interleukin-2 (IL-2). The JES6-1A12 antibody is a neutralizing antibody. Mouse IL-2 (also known as TCGF) is a 17 kDa factor produced mainly by activated CD4+ T cells. IL-2 induces cell cycle progression of resting cells in an antigen non-specific manner and allows clonal expansion of activated T cells. IL-2 also acts on activated B cells, monocytes, NK cells, LAK cells, and on oligodendroglial cells in vitro. In addition, IL-2 plays a role inhematopoiesis, tumor surveillance and anti-inflammatory reactions, and hence is a central regulator of the immune response. Non-glycosylated IL-2 is biologically active.Applications Reported: The JES6-1A12 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-2 bioactivity, and IP.Applications Tested: The Functional Grade Purified JES6-1A12 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-2 bioactivity.The JES6-1A12 antibody at 0.250 µg/mL has been found to inhibit by 50% the biological effects of 1.0 ng/mL mouse IL-2 in a CTLL-2 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.The JES6-1A12 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse Interleukin-2 (IL-2) in combination with the biotin JES6-5H4 (13-7021) antibody for detection and recombinant mouse IL-2 (14-8021) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/mL - 4 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL-4) and interleukin 7 (IL-7). The expression of the IL-2 gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a gene similar to IL-2 in mice leads to an ulcerative colitis-like disease that suggests an essential role of this gene in the immune response to antigenic stimuli.仅用于科研。不用于诊断过程。未经明确授权不得转售。

数据

IL-2 Antibody (16-7022-81) in ELISASplenocytes from mice pre-immunized with PLP139-151 peptide were recall activated 4 weeks later with PLP139-151 for 24 hours in Mouse IL-2 ELISPOT assay. Control well is medium alone.

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参考文献：

功能检测1.Nature immunologyThymic regulatory T cell niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition."16-7022 was used in Functional assays to investigate the sources of thymic IL-2 that limit access to the Treg developmental niche, showing that niche size is dictated by limiting IL-2 from antigen-bearing dendritic cells and feedback competition."AuthorsWeist BM,Kurd N,Boussier J,Chan SW,Robey EA2.Cell and bioscienceEvaluation of immunosuppressive function of regulatory T cells using a novel in vitro cytotoxicity assay."16-7022 was used in Functional assays to investigate the roles of regulatory T cells in treating various immune-mediated disorders."AuthorsZhang L,Manirarora JN,Wei CH3.PLoS pathogensThe systemic immune state of super-shedder mice is characterized by a unique neutrophil-dependent blunting of TH1 responses.中和4.European journal of immunologyTNF optimally activatives regulatory T cells by inducing TNF receptor superfamily members TNFR2, 4-1BB and OX40."16-7022 was used in Neutralization experiments to investigate pleiotropy in TNF inflammatory effects, showing that it activates Tregs by inducing TNFR2, 4-1BB and OX40."AuthorsHamano R,Huang J,Yoshimura T,Oppenheim JJ,Chen XELISA5.Nature communicationsCapicua deficiency induces autoimmunity and promotes follicular helper T cell differentiation via derepression of ETV5."Published figure using IL-2 monoclonal antibody (Product # 16-7022-81) in ELISA"

技术参数

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