IFN gamma Monoclonal Antibody (XMG1.2), Functional Grade, eBioscience™/IFN gamma 单克隆 (XMG1.2), 功能抗体, eBioscience™

货号：16-7311-81,16-7311-85,16-7311-38

规格：50 µg,500 µg,5 mg

价格：1081,3196,21777

产品类型：流式抗体

品牌：eBioscience

抗原：IFN gamma

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：XMG1.2

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（中和）；Assay-Dependent（功能检测）；

产品详细信息Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma. The XMG1.2 antibody is a neutralizing antibody. Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma.Glycosylation of IFN gamma does not affect its biological activity.Applications Tested: The Functional Grade Purified XMG1.2 antibody has been tested by LAL assay to verify low endotoxin levels and has been tested for ELISA capture and in bioassay for neutralization of IFN gamma bioactivity.The XMG1.2 antibody at 15 ng/mL has been found to inhibit by 50% the biological effects of 1 ng/mL of mouse IFN gamma (ND50), in an EMCV assay of L929 cell protection. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.The XMG1.2 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse IFN gamma in combination with the biotin R4-6A2 (13-7312) antibody for detection and recombinant mouse IFN gamma (39-8311) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.仅用于科研。不用于诊断过程。

数据

IFN gamma Antibody (16-7311-81)Intracellular staining of stimulated mouse splenocytes. As expected based on known expression patterns, IFN gamma clone XMG1.2 stains a minor subset of CD4+ T cells and a larger subset of NK1.1+ NK cells with no staining observed without stimulation. Details: Mouse splenocytes were cultured in the presence of Protein Transport Inhibitors (500X) (Unstimulated, bottom row) or Cell Stimulation Cocktail (plus protein transport inhibitors, 500X) for 5 hours (Stimulated, top row). Cells were fixed and permeabilized with the IC Fixation and Permeabilization Buffer Set and protocol followed by intracellular staining with CD4 (clone RM4-5), NK1.1 (clone PK136) and IFN gamma (clone XMG1.2). Cells in the CD4+ (blue histogram) or NK1.1+ (purple histogram) gates were used for analysis

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参考文献：

流式1.OncoimmunologyVectorization in an oncolytic vaccinia virus of an antibody, a Fab and a scFv against programmed cell death -1 (PD-1) allows their intratumoral delivery and an improved tumor-growth inhibition."16-7311 was used in Flow cytometry/Cell sorting to develop a method to vectorise a hamster monoclonal IgG against murine PD-1 in Western Reserve oncolytic vaccina virus."AuthorsKleinpeter P,Fend L,Thioudellet C,Geist M,Sfrontato N,Koerper V,Fahrner C,Schmitt D,Gantzer M,Remy-Ziller C,Brandely R,Villeval D,Rittner K,Silvestre N,Erbs P,Zitvogel L,Quéméneur E,Préville X,Marchand JB2.PloS onePreclinical Development of Ipilimumab and Nivolumab Combination Immunotherapy: Mouse Tumor Models, In Vitro Functional Studies, and Cynomolgus Macaque Toxicology."16-7311 was used in Flow cytometry/Cell sorting to exhibit a preclinical strategy for the identification and development of highly effective antitumour combination immunotherapies."AuthorsSelby MJ,Engelhardt JJ,Johnston RJ,Lu LS,Han M,Thudium K,Yao D,Quigley M,Valle J,Wang C,Chen B,Cardarelli PM,Blanset D,Korman AJ功能检测3.F1000ResearchMyelin-specific T helper 17 cells promote adult hippocampal neurogenesis through indirect mechanisms."16-7311 was used in Flow cytometry/Cell sorting to explore the proneurogenic potential of interleukin 17-producing T helper (Th17) cells."AuthorsNiebling J,E Rünker A,Schallenberg S,Kretschmer K,Kempermann G4.Clinical cancer research : an official journal of the American Association for Cancer ResearchCross-talk between T Cells and Hematopoietic Stem Cells during Adoptive Cellular Therapy for Malignant Glioma."16-7311 was used in a Functional assay to demonstrate that cellular interactions between adoptively transferred tumour-reactive T cells and bone marrow-derived haematopoietic stem and progenitor cells lead to the generation of potent intratumoural dendritic cells within the CNS compartment."AuthorsWildes TJ,Grippin A,Dyson KA,Wummer BM,Damiani DJ,Abraham RS,Flores CT,Mitchell DA中和5.Acta pharmacologica SinicaMycophenolic acid derivative 118 improves outcome of skin grafts by suppressing IL-17 production."16-7311 was used in Flow cytometry/Cell sorting to investigate the interaction between the mycophenolic acid derivative, 118, and the immune environment in a murine allogeneic skin graft model."AuthorsKong FY,Chen W,He SJ,Lin ZM,Li X,Zhang XH,Yang XQ,Zhu FH,Tong XK,Zhou Y,Tang W,Duan WH,Zuo JP

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