IL-1 beta Monoclonal Antibody (B122), Functional Grade, eBioscience™/IL-1 beta 单克隆 (B122), 功能抗体, eBioscience™

货号：16-7012-81,16-7012-85,16-7012-38,16-7012-025,16-7012-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1044,3274,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：IL-1 beta

物种：小鼠

宿主：Armenian hamster仓鼠

抗体亚型：IgG

克隆号：B122

荧光染料：其它

_ueditor_page_break_tag_

类型:	一抗(功能抗体）	同型对照：	Armenian Hamster IgG Isotype Control (eBio299Arm), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.125 µg/mL（ELISA）；Assay-Dependent（中和）；Assay-Dependent（功能检测）

产品详细信息Description: The B122 antibody reacts with mouse and rat interleukin-1beta (IL-1beta), a 17 kDa factor secreted primarily by monocytes. IL-1 has effects on T-helper cells, inducing IL-2 secretion and expression of IL-2 receptors.Applications Reported: The B122 antibody has been reported for use in cytokine neutralization and ELISA capture.Applications Tested: The Functional Grade Purified B122 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and inbioassay for neutralization of mouse IL-1 beta bioactivity.The B122 antibody at 0.125 µg/mL has been found to inhibit by 50% the biological effects of 4.0 ng/mL mouse IL-1 beta in a D10 cell proliferation assay. Detailed information and protocols about cytokine bioassays and in vitro cytokine neutralization using antibodies can be found in the BestProtocols® section.The B122 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-1 beta (IL-1 beta) in combination with the biotin anti-mouse Interleukin-1 beta (IL-1 beta) polyclonal (13-7112) antibody for detection and recombinant mouse IL-1 beta (14-8012) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin-1 beta (IL-1 beta) is a proinflammatory cytokine expressed by monocytes, macrophages, and dendritic cells. IL-1 beta is synthesized in response to inflammatory stimuli as a 31 kDa inactive pro-form that accumulates in the cytosol. Cleavage of pro-IL-1 beta into the active 17 kDa protein requires the activation of inflammasomes, which are multi-protein complexes that respond to pathogens, stress conditions, and other danger signals. Inflammasome activation triggers the processing of the caspase-1 precursor into its active form, which in turn cleaves pro-IL-1 beta. IL-1 beta lacks a signal sequence peptide for classical ER/Golgi pathway and is secreted alongside caspase-1 via an alternate and incompletely understood mechanism. Although IL-1 beta is most often secreted in its active form, secretion of the uncleaved protein may be detectable under some biological conditions. IL-1 beta signals through two receptors, IL-1RI and IL-1RII, both of which are shared with IL-1 alpha. IL-1 beta activity can be moderated by IL-1 Receptor Antagonist (IL-1RA), a protein produced by many cell types that blocks receptor binding through competitive inhibition. IL-1 beta play an important role in innate host defense by triggering the production of other proinflammatory cytokines in target cells and initiating acute-phase responses to infection and injury. Elevated levels of IL-1 beta have been associated with many chronic inflammatory conditions IL-1 beta neutralizing antibodies potential therapeutic value.仅用于科研。不用于诊断过程。

数据

IL-1 beta Antibody (16-7012-81)Nature communications 2015 -Figure 5 SENP1 deletion alters pancreatic adipocyte phenotype and augments NF-kappaB-dependent inflammation. ( a , b ) Pancreatic adipose was collected from 7 and 14 weeks old Ctrl and SENP1-aP2KO male (male, n =6). Morphology was visualized by haematoxylin and eosin stain staining. Scale bar, 20 mum ( a ). Cell sizes were quantified in ( b ). Three sections from each adipose tissue. Data are representative for three independent experiments. ( c ) Transcript levels of adipocyte differentiation markers (fatty acid synthase, adipose triglyceride lipase and lipoprotein lipase) in PATs were quantified by quantitative PCR with reverse transcription with GAPDH for normalization. n =6, male for each group. ( d ) Increased IKK-NF-kappaB activities in SENP1-aP2KO PATs. A representative western blot was from Ctrl and SENP1-aP2KO mice at the age of 7 weeks. Data are representative from three independent experiments is shown ( n =6, male). Relative protein levels were quantified from three blots by taking Ctrl as 1.0 ( n =6, male). ( e - h ) NF-kappaB activation is specifically detected in PATs of SENP1-aP2KO mice. ( e ) Phosphor-p65 staining (red) in PATs but not in pancreas. ( f ) Co-immunostaining of phosphor-p65 (green) and adipocyte marker FABP4 (red). ( g ) High-power images show co-staining of phosphor-p65 (red) in the nucleus of FABP4 + adipocytes (green). ( h ) Co-staining of phosphor-p65 (green) with APC-conjugated adipocyte marker FABP4 (green; yellow arrowheads), but not wit

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

WB1.IsletsThe lysine deacetylase inhibitor Givinostat inhibits β-cell IL-1β induced IL-1β transcription and processing."16-7012 was used in Western Blotting to demonstrate that inhibition of β-cell IL-1β expression and processing and Cxcl10 transcription contributes to the β-cell protective actions of KDACi."AuthorsDahllöf MS,Christensen DP,Lundh M,Dinarello CA,Mascagni P,Grunnet LG,Mandrup-Poulsen TELISA2.Nature communicationsSENP1-mediated NEMO deSUMOylation in adipocytes limits inflammatory responses and type-1 diabetes progression."Published figure using IL-1 beta monoclonal antibody (Product # 16-7012-81) in ELISA"中和3.PLoS pathogensCaspase-11 activation in response to bacterial secretion systems that access the host cytosol."16-7012 was used in Neutralization experiments to demonstrate that virulent bacteria trigger a rapid caspase-11-dependent innate immune response."AuthorsCasson CN,Copenhaver AM,Zwack EE,Nguyen HT,Strowig T,Javdan B,Bradley WP,Fung TC,Flavell RA,Brodsky IE,Shin S

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-26416.htm