IL-6 Monoclonal Antibody (MP5-20F3), Functional Grade, eBioscience™/IL-6 单克隆 (MP5-20F3), 功能抗体, eBioscience™

货号：16-7061-81,16-7061-85,16-7061-38

规格：50 µg,500 µg,5 mg

价格：1081,3389,20488

产品类型：流式抗体

品牌：eBioscience

抗原：IL-6

物种：小鼠

宿主：大鼠

抗体亚型：IgG1, kappa

克隆号：MP5-20F3

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5-2 µg/mL（ELISA）;Assay-Dependent(中和）；Assay-Dependent（功能检测）

产品详细信息Description: The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.Applications Reported: This MP5-20F3 antibody has been reported for use in cytokine neutralization, ELISA capture antibody, and ELISPOT capture antibody.Applications Tested: The Functional Grade Purified MP5-20F3 antibody has been tested by LAL assay to verify low endotoxin levels, by ELISA, and in bioassay for neutralization of mouse IL-6 bioactivity.The MP5-20F3 antibody at 0.03 µg/mL has been found to inhibit by 50% the biological effects of 0.01 ng/mL mouse IL-6 in an B9 cell proliferation assay. Detailed information and protocols about can be found in the BestProtocols® section.The MP5-20F3 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of mouse interleukin-6 (IL-6) in combination with the biotinylated MP5-32C11 (cat. 13-7062) antibody for detection and recombinant mouse IL-6 (cat. 14-8061) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8-1000 pg/mL should be included in each ELISA plate.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息Interleukin 6 (IL-6) is a multifunctional 26 kD protein originally discovered in the medium of RNA-stimulated fibroblastoid cells. IL-6 appears to be directly involved in the responses that occur after infection and cellular injury, and it may prove to be important as IL-1 and TNF-a in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages and endothelial cells. IL-6 acts upon a variety of cells including myeloidprogenitor cells, T cells, B cells, and hepatocytes. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors. IL-6 plays a critical role in B-cell differentiation to plasma cells and is a potent growth factor for plasmacytoma and myeloma. IL-6 is a very useful culture supplement for the generation of a high number of antibody-producing hybridomas. Primarily produced at sites of acute and chronic inflammation, IL-6 is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of IL-6 is implicated in a wide variety of inflammation-associated disease states including diabetes mellitus and systemic juvenile rheumatoid arthritis.仅用于科研。不用于诊断过程。

数据

IL-6 Antibody (16-7061-81) in ELISAMouse splenocytes (500,000 cells/well) were activated with LPS (1 µg/mL) for 48 hours in mouse IL-6 ELISPOT assay. Control well is medium alone.

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参考文献：

流式1.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and MetabolismDepletion of microglia exacerbates postischemic inflammation and brain injury."Published figure using IL-6 monoclonal antibody (Product # 16-7061-81) in Flow Cytometry"2.PloS oneReduced B lymphoid kinase (Blk) expression enhances proinflammatory cytokine production and induces nephrosis in C57BL/6-lpr/lpr mice."16-7061 was used in Flow cytometry/Cell sorting to demonstrate how BLK risk alleles confer susceptibility to systemic lupus erythematosus."AuthorsSamuelson EM,Laird RM,Papillion AM,Tatum AH,Princiotta MF,Hayes SM功能检测3.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsDistinct inflammatory signals have physiologically divergent effects on epigenetic regulation of Foxp3 expression and Treg function."16-7061 was used in Functional assays to investigate how different inflammatory signals affect Foxp3 expression and Tregs plasticity, showing that distinct signals have physiologically divergent effects on Foxp3 epigenetic regulation."AuthorsLal G,Yin N,Xu J,Lin M,Schroppel S,Ding Y,Marie I,Levy DE,Bromberg JS4.International immunologyIL-6 produced by immune complex-activated follicular dendritic cells promotes germinal center reactions, IgG responses and somatic hypermutation.中和5.Cell death and differentiationSprouty1 induces a senescence-associated secretory phenotype by regulating NFκB activity: implications for tumorigenesis."Published figure using IL-6 monoclonal antibody (Product # 16-7061-81) in Neutralization"

技术参数

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