TSLP Monoclonal Antibody (eBio28F12 (28F12)), Functional Grade, eBioscience™/TSLP 单克隆 (eBio28F12 (28F12)), 功能抗体, eBioscience™

货号：16-5491-82,16-5491-85,16-5491-38,16-5491-025,16-5491-050

规格：100 µg,500 µg,5 mg,25 mg,50 mg

价格：1315,3966,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：TSLP

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBio28F12 (28F12)

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The monoclonal antibody eBio28F12 reacts with and inhibits the bioactivity of mouse TSLP. Thymic Stromal-Derived Lymphopoetin (TSLP) was first identified in the tissue culture supernatant of a thymic stromal cell line as a growth factor capable of inducing proliferation and differentiation of pre-B cells. It was later found to be closely related to another stromal cytokine, IL-7, with which it shares overlapping functions. Along with its proliferative effects on B cells, TSLP induces dendritic cells to support the differentiation of naive T cells towards the Th2 lineage and may also be involved in the development of CD4^+CD25^+ regulatory T cells. The receptor for TSLP is heterodimeric and shares one subunit, IL-7Ralpha, with IL-7 and other members of the family, while the TSLPR subunit is unique to TSLP.TSLP expression can be induced by a variety of inflammatory cytokines and TLR ligands. Expression is regulated by NF-kappaB and has been detected in epithelial cells, stromal cells, and basophils. Elevated levels of TSLP are associated with asthma and atopic dermatitis, which are both Th2-mediated inflammatory conditions. It is believed that chronic overexpression of TSLP may result in increased sensitivity to allergens, resulting in susceptibility to these conditions.Applications Reported: The monoclonal antibody eBio28F12 reacts with and inhibits the bioactivity of mouse TSLP.Applications Tested: The ND50 of eBio28F12, as determined by the neutralization of recombinant mouse TSLP-induced proliferation of the Nag8/7 cell line, is 1-3 ng/mL in the presence of 0.5 ng/mL mTSLP. The neutralization dose of this antibody will vary depending on cell type, assay method, and cytokine concentration.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息TSLP is a 159 amino acid four helix-bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c (+) dendritic cells, naive CD4 (+), and CD8 (+) T cells into proallergic effectors, and primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation. TSLP influences the regulation of the positive selection of regulatory T cells and maintenance of peripheral CD4 (+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in the pathophysiology of inflammatory arthritis. It is expressed in heart, liver and prostate.仅用于科研。不用于诊断过程。

数据

TSLP Antibody (16-5491-82) in FNNeutralization of TSLP Recombinant Protein activity as measured by proliferation of Nag8/7 cells.

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参考文献：

ELISA1.eLifeEpidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility."16-5491 was used in Functional assays to investigate whether loss of structural proteins influences tumour formation."功能检测2.Toxicological sciences : an official journal of the Society of ToxicologyTriclosan Induces Thymic Stromal Lymphopoietin in Skin Promoting Th2 Allergic Responses."16-5491 was used in Functional assays to examine the effects of dermal triclosan exposure on the skin and lymph nodes of mice and in a human skin model to identify mechanisms for augmenting allergic responses."AuthorsMarshall NB,Lukomska E,Long CM,Kashon ML,Sharpnack DD,Nayak AP,Anderson KL,Jean Meade B,Anderson SE3.The Journal of clinical investigationAirway epithelial SPDEF integrates goblet cell differentiation and pulmonary Th2 inflammation."16-5491 was used in Functional assays to show that expression of SPDEF or FOXA3 in airway epithelial cells in neonatal mice caused airway hyperresponsiveness to methacholine."AuthorsRajavelu P,Chen G,Xu Y,Kitzmiller JA,Korfhagen TR,Whitsett JA4.eLifeEpidermal barrier defects link atopic dermatitis with altered skin cancer susceptibility."16-5491 was used in Functional assays to investigate whether loss of structural proteins influences tumour formation."中和5.ImmunologyInterleukin-4- and NACHT, LRR and PYD domains-containing protein 3-independent mechanisms of alum enhanced T helper type 2 responses on basophils."Published figure using TSLP monoclonal antibody (Product # 16-5491-82) in Neutralization"

技术参数

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