Asialo GM1 Polyclonal Antibody, Functional Grade, eBioscience™/Asialo GM1 多克隆, 功能抗体, eBioscience™

货号：16-6507-39,16-6507-38,16-6507-025,16-6507-050

规格：1 mg,5 mg,25 mg,50 mg

价格：5725,0,0,0

产品类型：流式抗体

品牌：eBioscience

抗原：Asialo GM1

物种：人/小鼠/大鼠

宿主：兔

抗体亚型：IgG

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	IgG
浓度：	冻干粉	用法：	Assay-Dependent（IF）;Assay-Dependent（Flow）;Assay-Dependent（功能检测）

产品详细信息Description: This rabbit polyclonal antibody reacts with the asialo ganglio-N-tetraosylceramide (asialo-GM1), a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression. The antibody recognizes asialo-GM1 specifically and not other glycolipids, including GM1 and asialo-GM2.This antibody has been reported to deplete NK cells in mice and hamsters in vivo and exhibits in vitro effects in a variety of species.This polyclonal antibody has been reported for use in flow cytometric analysis, depletion of NK cells, and in vitro functional studies. Intravenous and IP injection of 20-50 µL has been shown to decrease NK1.1 populations after 3-4 days. Repeat injections may be necessary.Applications Tested: This polyclonal antibody against asialo-GM1 has been tested by flow cytometric analysis of mouse splenocytes. Optimal staining concentration will vary depending on the lot. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/µg antibody as determined by the LAL assay.Storage and handling: It is recommended to add 1 mL of sterile (endotoxin free) room temperature water. After reconstitution pass through 0.22 µm filter prior to injection.靶标信息Asialo ganglio-N-tetraosylceramide (asialo-GM1) is a glycolipid on human, mouse, rat, bovine and hamster cells. Glycolipids such as this sialyated version are defined by the specific transferases that modify the lipid. Glycolipids are targets of immune cell response. Auto-antibodies have also been identified. Additionally these glycolipids can act as co-receptors for pathogens. Asialo-GM1 is present on both NK cells and a subset of monocyte/macrophages from the mouse spleen. Expression is also found on rat and bovine brain, fetal liver and on fetal immature thymocytes. Expression has also been shown to increase with tumor progression.仅用于科研。不用于诊断过程。

数据

Asialo GM1 Antibody (16-6507-39) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse NK1-1 PerCP-Cy5-5 (Product # 45-5941-82) (left) or Anti-Mouse CD11b APC (Product # 17-0112-82) (right) and Anti-Asialo-GM1 Functional Grade Purified.An Anti-Rabbit PE (Product # 12-4739-81) was used for detection.Total viable cells were used for analysis.

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参考文献：

流式1.EMBO molecular medicineProlonged contact with dendritic cells turns lymph node-resident NK cells into anti-tumor effectors."16-6507 was used in Flow cytometry/Cell sorting to understand natural killer cell activation dynamics, interactions with accessory cells, and trafficking between secondary lymphoid organs and tumoural tissues."AuthorsMingozzi F,Spreafico R,Gorletta T,Cigni C,Di Gioia M,Caccia M,Sironi L,Collini M,Soncini M,Rusconi M,von Andrian UH,Chirico G,Zanoni I,Granucci F功能检测2.Scientific reportsInterleukin-37 mediates the antitumor activity in hepatocellular carcinoma: role for CD57+ NK cells."16-6507 was used in Neutralization experiments to investigate the role of IL-37 in cancer."AuthorsZhao JJ,Pan QZ,Pan K,Weng DS,Wang QJ,Li JJ,Lv L,Wang DD,Zheng HX,Jiang SS,Zhang XF,Xia JC3.PLoS pathogensBlock of death-receptor apoptosis protects mouse cytomegalovirus from macrophages and is a determinant of virulence in immunodeficient hosts."16-6507 was used in Neutralization experiments to investigate the role of death-receptor blockade in cytomegalovirus infection."AuthorsEbermann L,Ruzsics Z,Guzmán CA,van Rooijen N,Casalegno-Garduño R,Koszinowski U,Čičin-Šain L中和4.CancersTargeted Vaccination against Human α-Lactalbumin for Immunotherapy and Primary Immunoprevention of Triple Negative Breast Cancer."16-6507 was used in in vivo experiments to investigate the use of retired self-proteins in the treatment of cancer, showing that vaccination against α-Lactalbumin can be used against triple negative breast cancers."AuthorsTuohy VK,Jaini R,Johnson JM,Loya MG,Wilk D,Downs-Kelly E,Mazumder S

技术参数

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