CD8a Monoclonal Antibody (53-6.7), Functional Grade, eBioscience™/CD8a 单克隆 (53-6.7), 功能抗体, eBioscience™
货号：16-0081-81,16-0081-82,16-0081-85,16-0081-86,16-0081-38,16-0081-025,16-0081-050
规格：50 µg,100 µg,500 µg,1mg,5 mg,25 mg,50 mg
价格：934,1369,2636,4818,24089,72266,120443
产品类型：流式抗体
品牌：eBioscience
抗原：CD8a
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：53-6.7
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 0.25 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测） |
产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis. It has also been reported in T cell activation, isolation and depletion of CD8+ cells, and blocking of ligand binding.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.仅用于科研。不用于诊断过程。
数据 |
| CD8a Antibody (16-0081-82)Cell regeneration (London, England) 2018 -Fig. 2 Flow cytometry analysis of the purity of sorted Ter119 - Mac1 - CD3 - CD4 - CD8 - B220 + CD19 + CD93 + IgM - pro/pre-B cells . Bone marrow nucleated cells were first incubated with biotin-conjugated anti-B220 antibody and then enriched by streptavidin microbeads using AutoMACS Pro. The pro/pre-B cells were further sorted from the B220 enriched cells by defined markers. Flow cytometry analysis confirmed the purities of B220 enriched cells (top) and sorted pro/pre-B cells (bottom). |
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参考文献： |
| 流式1.Frontiers in neurologyIL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury."Published figure using CD8a monoclonal antibody (Product # 16-0081-82) in Flow Cytometry"2.Frontiers in physiologyMacrophage Depletion Protects against Cigarette Smoke-Induced Inflammatory Response in the Mouse Colon and Lung."Published figure using CD8a monoclonal antibody (Product # 16-0081-82) in Flow Cytometry"功能检测3.The Journal of clinical investigationAdipose tissue B2 cells promote insulin resistance through leukotriene LTB4/LTB4R1 signaling."Published figure using CD8a monoclonal antibody (Product # 16-0081-82) in Flow Cytometry"4. Human vaccines and immunotherapeuticsComparative immunogenicity in mice of rotavirus VP6 tubular structures and virus-like particles."16-0081 was used in Functional assays to compare immunogenicity of rotavirus VP6 tubular structures and virus-like particles in mice."AuthorsLappalainen S,Tamminen K,Vesikari T,Blazevic VELISA5.PloS oneProtective immunity against lethal F. tularensis holarctica LVS provided by vaccination with selected novel CD8+ T cell epitopes."16-0081 was used in an ELISpot assay to demonstrate a novel method for bacterial clearance involving the induction of an immune response to specific pathogenic epitopes."AuthorsRotem S,Cohen O,Bar-Haim E,Bar-On L,Ehrlich S,Shafferman A6.PloS oneClusters versus affinity-based approaches in F. tularensis whole genome search of CTL epitopes."16-0081 was used in an ELISpot assay to suggest that a cluster-based approach can be valuable in identification of novel CTL epitopes in proteomes."AuthorsZvi A,Rotem S,Cohen O,Shafferman A |
QQ:1749072012