CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PE, eBioscience™

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CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PE, eBioscience™

货号:12-9987-82,12-9987-80

规格:100 µg,25 µg

价格:2271,925

产品类型:流式抗体

品牌:eBioscience

抗原:CD235a

物种:人

宿主:小鼠

抗体亚型:IgG2b, kappa

克隆号:HIR2 (GA-R2)

荧光染料:PE

类型:

单抗

同型对照:

Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

免疫原性:

用法:

0.015 µg/test(Flow)

Product Specific InformationDescription: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationGlycophorins A (GPA) and B (GPB) which are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD235a (Glycophorin A) Antibody (12-9987-82) in FlowStaining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control PE (Product # 12-4732-81) (blue histogram) or Anti-Human CD235a (Glycophorin A) PE (purple histogram). Non-leukocytes were used for analysis.

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参考文献:

1.JCI insightDimethyl fumarate increases fetal hemoglobin, provides heme detoxification, and corrects anemia in sickle cell disease.AuthorsKrishnamoorthy S,Pace B,Gupta D,Sturtevant S,Li B,Makala L,Brittain J,Moore N,Vieira BF,Thullen T,Stone I,Li H,Hobbs WE,Light DR

2.PloS oneKnockdown of HSPA9 induces TP53-dependent apoptosis in human hematopoietic progenitor cells."12-9987 was used in Flow cytometry/Cell sorting to indicate that reduced levels of HSPA9 may contribute to TP53 activation and increased apoptosis in del(5q)-associated myelodysplastic syndromes."AuthorsLiu T,Krysiak K,Shirai CL,Kim S,Shao J,Ndonwi M,Walter MJ

3.Scientific reportsPlatelets confound the measurement of extracellular miRNA in archived plasma.

4.Scientific reportsASXL1 plays an important role in erythropoiesis."12-9987 was used in Flow cytometry/Cell sorting to unveil a key role for ASXL1 in erythropoiesis and indicate that ASXL1 loss hinders erythroid development/maturation."AuthorsShi H,Yamamoto S,Sheng M,Bai J,Zhang P,Chen R,Chen S,Shi L,Abdel-Wahab O,Xu M,Zhou Y,Yang FC

5.PloS onePDL1 Expression on Plasma and Dendritic Cells in Myeloma Bone Marrow Suggests Benefit of Targeted anti PD1-PDL1 Therapy."12-9987 was used in Flow cytometry/Cell sorting to investigate whether anti PDL1 or PD-1 treatment targeting the immune system could be used against multiple myeloma."AuthorsSponaas AM,Moharrami NN,Feyzi E,Standal T,Holth Rustad E,Waage A,Sundan A

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