CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience™/CD54 (ICAM-1) 单克隆 (YN1/1.7.4), 功能抗体, eBioscience™

货号：16-0541-81,16-0541-85,16-0541-38,16-0541-025,16-0541-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1156,3496,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD54

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：YN1/1.7.4

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis. YN1/1.7.4 has also been reported in blocking of CD54 in functional studies.Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.仅用于科研。不用于诊断过程。

数据

CD54 (ICAM-1) Antibody (16-0541-81) in FlowStaining of mouse splenocytes with Anti-Mouse CD54 (ICAM-1) FITC (left) or PE (right). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

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参考文献：

流式1.International journal of molecular sciencesLow-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry"2.Cell stem cellConstitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry"3.Scientific reportsFibroblastic reticular cells of the lymphoid tissues modulate T cell activation threshold during homeostasis via hyperactive cyclooxygenase-2/prostaglandin E2axis."16-0541 was used in Flow cytometry/Cell sorting to identify an intrinsic mechanism of fibroblastic reticular cells shared between mice and humans for suppressing T cell sensitivity to activation via PGE2."AuthorsYu M,Guo G,Zhang X,Li L,Yang W,Bollag R,Cui Y功能检测4.The Journal of investigative dermatologyNatural killer T cells are essential for the development of contact hypersensitivity in BALB/c mice."16-0541 was used in Flow cytometry/Cell sorting to report the pivotal contribution of dendritic and natural killer T cell interactions to hypersensitivity."AuthorsShimizuhira C,Otsuka A,Honda T,Kitoh A,Egawa G,Nakajima S,Nakashima C,Watarai H,Miyachi Y,Kabashima KIF5.Nature communicationsPalmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome."Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Immunofluorescence"中和6.Nature communicationsTreg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration."16-0541 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration."AuthorsBrinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

技术参数

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