CD172a (SIRP alpha) Monoclonal Antibody (P84), Functional Grade, eBioscience™/CD172a (SIRP alpha) 单克隆 (P84), 功能抗体, eBioscience™

货号：待删除,16-1721-81

规格：500 µg,50 µg

价格：4228,1813

产品类型：流式抗体

品牌：eBioscience

抗原：CD172a

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P84

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Rat IgG1 kappa Isotype Control (eBRG1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.25 µg/test（Flow）；Assay-Dependent（FN）

产品详细信息Description: This P84 monoclonal antibody reacts with mouse CD172a, also known as signal regulatory protein a (SIRPa). This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, macrophages, dendritic cells, but not on T and B lymphocytes. Moreover, neurons and other tissues of the central nervous system have also been shown to express CD172a. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.This monoclonal antibody has been reported to have neutralizing activity.Applications Reported: This P84 antibody has been reported for use in flow cytometric analysis and neutralization assays.Applications Tested: This P84 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth.仅用于科研。不用于诊断过程。

数据

CD172a (SIRP alpha) Antibody (16-1721-85)Multiple sclerosis (Houndmills, Basingstoke, England) 2012 -Figure 7. Confirmation of envelope (Env) expression in perivascular macrophages by double Immunostaining. Sections were selected from a lesion area including a vascular element with evidence of perivascular macrophage cuffs infiltrating the parenchyma, as in Figure 6D . (A) Perivascular macrophages labelled with anti-Env GN-mAb_05 mouse monoclonal (1:100) and revealed in green with fluorescent labelled secondary antibodies; (B) immunoactive macrophage labelled with anti-MHC class II LN3 antibody (1:50) and revealed in red with fluorescent labelled secondary antibodies (same slide); (C) merge of (A) and (B) immunolabelling evidencing the co-localization of both anti-Env and anti-MHC II antigens in macrophage cells from a perivascular cuff, in the same sample from an active multiple sclerosis lesion. In cells where the two antigens are co-expressed, orange, yellow or light-brown colour is seen when the two antigens have superimposed location with varying proportion, thus modulating the merged colours. Few cells also seem to express a single antigen, with single colour.

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参考文献：

流式1.European journal of immunologyRevisiting the specificity of the MHC class¿II transactivator CIITA in classical murine dendritic cells in vivo."16-1721 was used in Flow cytometry/Cell sorting to find that CIITA exerts a highly restricted control over only the MHCII, H2-DO and H2-DM genes, in DC1 and DC2 cDC subsets, but not over other proposed targets, including Ii."AuthorsAnderson DA,Grajales-Reyes GE,Satpathy AT,Vasquez Hueichucura CE,Murphy TL,Murphy KM2.The Journal of experimental medicineDll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice."16-1721 was used in Flow cytometry/Cell sorting to investigate how Dll4-Notch signaling affects pro-T cell fate and thymic DC development, showing that it is a novel Flt3-independent pathway for DC development and autoimmunity."AuthorsBilliard F,Lobry C,Darrasse-Jèze G,Waite J,Liu X,Mouquet H,DaNave A,Tait M,Idoyaga J,Leboeuf M,Kyratsous CA,Burton J,Kalter J,Klinakis A,Zhang W,Thurston G,Merad M,Steinman RM,Murphy AJ,Yancopoulos GD,Aifantis I,Skokos DIF3.Journal of visualized experiments : JoVEPrecision-cut Mouse Lung Slices to Visualize Live Pulmonary Dendritic Cells."Published figure using CD172a (SIRP alpha) monoclonal antibody (Product # 16-1721-81) in Immunofluorescence"4.Multiple sclerosis (Houndmills, Basingstoke, England)Human endogenous retrovirus type W envelope expression in blood and brain cells provides new insights into multiple sclerosis disease."Published figure using CD172a (SIRP alpha) monoclonal antibody (Product # 16-1721-81) in Immunofluorescence"

技术参数

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