CD115 (c-fms) Monoclonal Antibody (AFS98), Functional Grade, eBioscience™/CD115 (c-fms)单克隆 (AFS98),功能抗体, eBioscience™

货号：16-1152-82,16-1152-38,16-1152-025,16-1152-050

规格：100 µg,5 mg,25 mg,50 mg

价格：2564,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD115 (c-fms)

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：AFS98

荧光染料：其它

_ueditor_page_break_tag_

类型:	一抗(功能抗体）	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）;Assay-Dependent（中和）;Assay-Dependent（功能检测）

产品详细信息Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.Applications Reported: The AFS98 antibodyhas been reported for use in flow cytometric analysis. It has also been reported in blocking of ligand binding.Applications Tested: The AFS98 antibody has been tested by blocking of fluorochrome conjugated AFS98 in flow cytometric analysis of peritoneal exudate cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CSF1R (colony stimulating factor 1 receptor) is a cytokine that controls the production, differentiation, and function of macrophages. CSF1R mediates most if not all of the biological effects of CSF1. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.仅用于科研。不用于诊断过程。

数据

CD115 (c-fms) Antibody (16-1152-82) in FlowSurface staining of thioglycolate-induced peritoneal exudate cells (PECs) with Anti-Mouse CD115 (c-fms) PE. Appropriate isotype controls were used (open histogram). Cells in the large scatter population were used for analysis.

推荐产品：

二抗荧光二抗免疫组化一抗标签抗体

参考文献：

流式1.Journal of diabetes researchAdvanced Glycation End Products Enhance Murine Monocyte Proliferation in Bone Marrow and Prime Them into an Inflammatory Phenotype through MAPK Signaling."Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry"2.Journal of cellular physiologyBone loss in C57BL/6J-OlaHsd mice, a substrain of C57BL/6J carrying mutated alpha-synuclein and multimerin-1 genes."Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Flow Cytometry"IF3.BloodEndothelial cells provide an instructive niche for the differentiation and functional polarization of M2-like macrophages."Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunofluorescence"4.The Journal of clinical investigationStat3-dependent acute Rantes production in vascular smooth muscle cells modulates inflammation following arterial injury in mice."Published figure using CD115 (c-fms) monoclonal antibody (Product # 16-1152-82) in Immunofluorescence"中和5.The American journal of pathologyAntibiotic-Induced Dysbiosis of Gut Microbiota Impairs Corneal Nerve Regeneration by Affecting CCR2-Negative Macrophage Distribution."16-1152 was used in Immunodepletion to study the relationship between dysbiosis of the gut microbiota induced by antibiotic treatment and impaired corneal nerve regeneration."AuthorsLiu J,Wu M,He J,Xiao C,Xue Y,Fu T,Lin C,Dong D,Li Z

技术参数

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-26537.htm