Antibody Isotyping 7-Plex Mouse ProcartaPlex Panel 2

货号：EPX070-20816-901

规格：96 tests

价格：1

产品类型：多因子检测

品牌：Thermo Fisher

物种：人

宿主：其它

抗体亚型：其它

荧光染料：其它

测定范围:	See Certificate of Analysis
样品体积:	Serum, Plasma: 25 µL; CCS: 50 µL
检测间 CV:	Less than 15%
检测内 CV:	Less than 15%
数量:	96 tests
目标种属:	Mouse
样品类型:	Serum, Plasma, Cell Culture Supernatants
多路传输尺寸重叠数量:	7
运输条件:	Wet Ice
产品线:	ProcartaPlex
免疫测定试剂盒格式:	Multiplex Kit
适用于（设备）:	Luminex Instruments

The Mouse Antibody Isotyping 7-Plex ProcartaPlex Panel 2 enables determination of antibody heavy chain subclasses by analyzing 7 protein targets using Luminex xMAP technology. Antibody isotyping is a critical and beneficial tool in hybridoma development. Simultaneous detection of these targets can help researchers explore analysis of immunoglobulin isotypes during and after hybridoma development and to assess immune responses. The panel is provided in a ready-to-use format with individual vials of capture and detection reagents formulated at 1X concentration, requiring less pipetting and experimental setup. It is not combinable with simplexes or other panels.ProcartaPlex preconfigured panels are extensively tested for analyte combinability, interference, and cross-reactivity to provide the highest level of validation and precision. All ProcartaPlex panels are supplied with the necessary reagents to perform the assay.ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine). Visit ourProcartaPlex Immunoassays pagefor more information and available products.Target list [bead region]:IgA [38], IgE [42], IgG1 [12], IgG2b [21], IgG2c [43], IgG3 [25], IgM [52]Recommended sample dilution:Serum, Plasma: 1:40000; Cell culture supernatant: 1:200About ProcartaPlex assays for the Luminex platformProcartaPlex immunoassays are based on the principles of a sandwich ELISA, using two highly specific antibodies binding to different epitopes of one protein to quantitate all protein targets simultaneously using a Luminex instrument. ProcartaPlex multiplex assays require as little as 25 µL of plasma or serum, or 50 µL of cell culture supernatant, and just four hours to obtain analyzed results.Features include:• Reproducible, reliable results—validated as a panel to the highest industry standard, including protein target combinability and cross-reactivity testing• More results per sample—measure multiple protein targets simultaneously in a single 25–50 µL sample• Well-established Luminex technology highly referenced multiplexing platform for protein detection and quantitationProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for the simultaneous detection and quantitation of up to 65 protein targets in a single 25–50 µL sample—from plasma, serum, cell culture supernatants, and other bodily fluids.The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores to create spectrally unique signatures that can be identified by the Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, and MAGPIX). Similar to a sandwich ELISA, the ProcartaPlex assay uses matched antibody pairs to identify the protein of interest. In a multiplexed assay, each spectrally unique bead is labeled with antibodies specific for a single target protein, and bound proteins are identified with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). The conjugation of protein-specific antibodies to a distinct bead allows for analysis of multiple targets in a single well.The most significant difference between a ProcartaPlex assay and ELISA is that the capture antibody in the ProcartaPlex assay is conjugated to a bead and not adsorbed to the microplate well, so the ProcartaPlex assay reagents are free-floating in the solution. For detection, the Luminex 200 instrument, for example, contains two lasers, one to distinguish the spectral signature of each bead and the second to quantify the amount of RPE fluorescence, which is proportional to the amount of protein present in the sample. ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time that it takes to perform a traditional sandwich ELISA.

技术参数

组成成分 • 2 vials Mouse Isotyping Standard Mix (lyophilized)• 2 vials Mouse IgG2c Standard (lyophilized)• 1 vial Capture Bead Mix (1X)• 1 vial Biotinylated Detection Antibody Mix (50X)• 1 bottle Reading Buffer (1X)• 1 bottle Wash Buffer (10x)• 1 bottle Universal Assay Buffer (10x)• 1 bottle Detection Antibody Diluent (1X)• 8-tube strip• Adhesive film• Flat-bottom 96-well plate, black

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