CD11a (LFA-1alpha) Monoclonal Antibody (HI111), Functional Grade, eBioscience™/CD11a (LFA-1alpha) 单克隆 (HI111), 功能抗体, eBioscience™

货号：16-0119-81,16-0119-025,16-0119-050

规格：50 µg,25 mg,50 mg

价格：1283,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD11a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：HI111

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	1 µg/test（Flow）；Assay-Dependent（Neu）；Assay-Dependent（FN）

产品详细信息Description: The HI111 monoclonal antibody reacts with human CD11a, the 180 kDa integrin alphaL, also known as the lymphocyte function associated antigen-1 (LFA-1) alpha chain. LFA-1, formed by non-covalent association of CD11a with CD18 (integrin beta2), serves as an important adhesion molecule involved in lymphocyte and granulocyte function. CD54 (ICAM-1), CD102 (ICAM-2), and CD50 (ICAM-3) are ligands for LFA-1. CD11a is expressed by all leukocytes.Applications Reported: The HI111 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CD11a function in mixed lymphocyte reactions.Applications Tested: The HI111 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD11a (LFA-1 alpha, ITGAL) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. CD11a plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. CD11a is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of CD11a to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. CD11a is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of CD11a function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti- CD11a antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. Two transcript variants encoding different isoforms of CD11a have been found. Diseases associated with CD11a dysfunction include benign cephalic histiocytosis and leukocyte adhesion deficiency.仅用于科研。不用于诊断过程。

数据

CD11a (LFA-1alpha) Antibody (16-0119-81) in FlowStaining of normal human peripheral blood with Anti-Human CD11a Functional Grade Purified and detected with Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were analyzed.

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参考文献：

流式1.Journal of leukocyte biologyHuman scavenger protein AIM increases foam cell formation and CD36-mediated oxLDL uptake."16-0119 was used in Flow cytometry/Cell sorting to study how hAIM is involved in macrophage survival, adhesion, and foam cell formation, and can serve to facilitate CD36-mediated uptake of oxLDL."AuthorsAmézaga N,Sanjurjo L,Julve J,Aran G,Pérez-Cabezas B,Bastos-Amador P,Armengol C,Vilella R,Escolà-Gil JC,Blanco-Vaca F,Borràs FE,Valledor AF,Sarrias MR2.RetrovirologyHIV transfer between CD4 T cells does not require LFA-1 binding to ICAM-1 and is governed by the interaction of HIV envelope glycoprotein with CD4."16-0119 was used in Flow cytometry/Cell sorting to evaluate the role of LFA-1 and its ligands in viral transmission between HIV-producing cells and primary CD4 T-cells."AuthorsPuigdomènech I,Massanella M,Izquierdo-Useros N,Ruiz-Hernandez R,Curriu M,Bofill M,Martinez-Picado J,Juan M,Clotet B,Blanco J中和3.Journal of immunology (Baltimore, Md. : 1950)Identification of endothelial cell junctional proteins and lymphocyte receptors involved in transendothelial migration of human effector memory CD4+ T cells."16-0119 was used in Blocking experiments to further analyze effector memory (EM) CD4(+) T cell migrational responses to either chemokine or TCR-activating signals."AuthorsManes TD,Pober JSICC4. Biophysical journalMembrane mobility of beta2 integrins and rolling associated adhesion molecules in resting neutrophils."16-0119 was used in Immunofluorescence to present an analytical model to measure the mobility of the four key receptors involved in the neutrophil adhesion cascade."AuthorsGaborski TR,Clark A,Waugh RE,McGrath JLIF5.Biophysical journalMembrane mobility of beta2 integrins and rolling associated adhesion molecules in resting neutrophils."16-0119 was used in Immunofluorescence to present an analytical model to measure the mobility of the four key receptors involved in the neutrophil adhesion cascade."AuthorsGaborski TR,Clark A,Waugh RE,McGrath JL

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