Pro-Detect Rapid Antibody Isotyping Assay Kit, rat
货号：A38551
规格：10 tests
价格：2493
产品类型：检测试剂盒
品牌：Thermo Fisher
物种：大鼠
宿主：其它
抗体亚型：其它
荧光染料：其它
| The Thermo Scientific Pro-Detect Rapid Antibody Isotyping Assay Kit - Rat is a single-use, dipstick lateral flow kit for quick, easy determination of rat monoclonal antibody class and subclass identity. This antibody isotyping assay uses membrane-based strips coated with dye-bound conjugated antibodies that provide a visual, color readout of the monoclonal antibody isotype within ten minutes. The kit determines rat IgG1, IgG2a, IgG2b , and IgG2c.Each Pro-Detect Rapid Antibody Isotyping assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to a test tube or microplate well and dipping in a lateral flow strip. Gold conjugates embedded in the strip membrane form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated regions of the membrane. Results are displayed as a red band above the printed class or sub-class description, indicating the antibody isotype present.Determining the subclass of a rat antibody is important when selecting the best purification method. IgG2a, IgG2b, and IgG2c can be purified with Protein A columns at a pH of 7–8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8–9.The Pro-Detect Rapid Antibody Isotyping Assay Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex bead-based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5 µL ascites fluid, 2–22 µL of cell culture supernatant or 1.5 ng of purified antibody. |
| 特点 |
| •Long shelf life—stable for at least one year at 4°C•Simple workflow—dilute antibody sample using provided diluent and dip in the lateral flow strip•Fast—within 5–10 minutes a band appears that indicates the antibody isotype•Sensitive—detect antibodies in tissue culture media or ascites fluid samples down to low ng/mL concentrations•Specific—assay does not cross-react with fetal bovine serum (FBS)•Reliable—results are similar to standard ELISA-based isotyping assays |
技术参数 组成成分 • 10 Rapid Antibody Isotyping Assay Strips 1• 10 Rapid Antibody Isotyping Assay Strips 2• Sample Diluent, 15 mLSufficient for 10 isotype determinationsStore at 2–8°C.
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