Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin
货号：13-4732-85
规格：100μg
价格：1545
产品类型：小鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：Biotin
| 抗体类型 | | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | Assay-Dependent(IHC)；Assay-Dependent(ICC)；Assay-Dependent (IF)；Assay-Dependent(Flow) |
| 产品详细信息Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.Applications Reported: This mouse IgG2b isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This mouse IgG2b isotype control has been tested by flow cytometric analysis of human peripheral blood leukocytes and shoudl be used at the ssame concentration as the experimental antibody.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| Mouse IgG2b kappa Isotype Control (13-4732-85) in IHC (P)Immunohistochemistry of formalin-fixed paraffin embedded human breast cancer tissue using 20 µg/mL Mouse IgG2b Isotype Control Biotin (left) or 20 µg/mL Anti-Human/Mouse ErbB4 Biotin (right), followed by streptavidin HRP and DAB visualization. Nuclei are counterstained with hematoxylin. |
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| 参考文献： |
| 1.Frontiers in physiologyRed Cell Properties after Different Modes of Blood Transportation."13-4732 was used in an ELISA assay to demonstrate that different red blood cell samples may require different shipment settings (including anticoagulants, temperature, and timing)."AuthorsMakhro A,Huisjes R,Verhagen LP,Mañú-Pereira Mdel M,Llaudet-Planas E,Petkova-Kirova P,Wang J,Eichler H,Bogdanova A,van Wijk R,Vives-Corrons JL,Kaestner L |
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