CD184 (CXCR4) Monoclonal Antibody (12G5), Functional Grade, eBioscience™/CD184 (CXCR4) 单克隆 (12G5), 功能抗体, eBioscience™

货号：16-9999-81,16-9999-85,16-9999-38,16-9999-025,16-9999-050

规格：50 µg,500 µg,5 mg,25 mg,50 mg

价格：1752,4324,24089,72266,120443

产品类型：流式抗体

品牌：eBioscience

抗原：CD184 (CXCR4)

物种：人

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：12G5

荧光染料：其它

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类型:	一抗(功能抗体）	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™
浓度：	1 mg/mL	用法：	0.5 µg/test（Flow）;Assay-Dependent（功能检测）;Assay-Dependent（中和）

产品详细信息Description: The 12G5 monoclonal antibody reacts with human CD184 (CXCR4), also termed Fusin, LESTR, or HUMSTR. CD184 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a coreceptor for X4 HIV-1 entry into CD4+ cells. CD184 is expressed predominantly on naive T cell subsets of peripheral blood and is rapidly upregulated by PHA and IL-2 stimulation. The 12G5 antibody shows partial inhibition of chemotaxis and calcium influx induced by SDF-1 (the natural ligand of CD184), blocks CD4-independent HIV-2 infection, and blocks CD4-dependent infection by some T-tropic HIV-1 isolates.Applications Reported: The 12G5 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of CXCR4 in functional studies. (Fluorochrome-conjugated 12G5 is recommended for use in flow cytometry).Applications Tested: The 12G5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.仅用于科研。不用于诊断过程。

数据

CD184 (CXCR4) Antibody (16-9999-81) in FlowStaining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD184 (CXCR4) PE-Cyanine5 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

中和1.Virology journalHuman cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts."Published figure using CD184 (CXCR4) monoclonal antibody (Product # 16-9999-81) in Neutralization"功能检测2.PloS oneOsteopontin enhances the expression and activity of MMP-2 via the SDF-1/CXCR4 axis in hepatocellular carcinoma cell lines.流式3.Stem cells (Dayton, Ohio)ST8SIA4-Dependent Polysialylation is Part of a Developmental Program Required for Germ Layer Formation from Human Pluripotent Stem Cells."Published figure using CD184 (CXCR4) monoclonal antibody (Product # 16-9999-81) in Flow Cytometry"WB4.BiochemistryAntibody epitopes on g protein-coupled receptors mapped with genetically encoded photoactivatable cross-linkers."Published figure using CD184 (CXCR4) monoclonal antibody (Product # 16-9999-81) in Western Blot"5.Virology journalHuman cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts."Published figure using CD184 (CXCR4) monoclonal antibody (Product # 16-9999-81) in Neutralization"

技术参数

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