CD317 (BST2, PDCA-1) Monoclonal Antibody (26F8), Functional Grade, eBioscience™/CD317 (BST2, PDCA-1) 单克隆 (26F8), 功能抗体, eBioscience™
货号：16-3179-82,16-3179-025,16-3179-050
规格：100 µg,25 mg,50 mg
价格：3034,72266,120443
产品类型：流式抗体
品牌：eBioscience
抗原：CD317 (BST2, PDCA-1)
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：26F8
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Functional Grade, eBioscience™ |
浓度： | 1 mg/mL | 用法： | 0.125 µg/test（Flow）;Assay-Dependent（功能检测）;Assay-Dependent（中和） |
产品详细信息Description: This 26F8 monoclonal antibody reacts with human CD317 (also known as BST2 and tetherin). CD317 is a 30-36-kDa type II transmembrane protein expressed on B cells and bone marrow stromal cells. Although reports have indicated that CD317 mRNA is detectable in activated T cells, protein expression in primary T cells and macrophages is undetectable. Moreover, certain T cell lines, such as Jurkat, do not express detectable levels of CD317 protein. CD317 has been associated with pre-B cellgrowth and the terminal differentiation of plasma B cells. More recently, this molecule has been reported to prevent HIV-1 virion release from the surface of infected cells, leading to reuptake and degradation of the virus. This activity is inhibited by the HIV-1 accessory protein Vpu. CD317 has been identified as the ligand for the ILT7 receptor.Applications Reported: This 26F8 antibody has been reported for use in flow cytometric analysis and functional assays. The 26F8 clone has been reported to block the binding of CD317 to the ILT7 receptor.Applications Tested: This 26F8 antibody has been tested by flow cytometric analysis on MCF7 cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息BST2 (CD317, HM1.24 antigen, DAMP-2, tetherin) is an integral membrane protein that is involved in the development and growth of B-cells. The surface expression of BST2 on fibroblast cell lines facilitated the stromal cell-dependent growth of DW34, a pre-B-cell line. BST2 is highly expressed during B-cell development, from pro-B precursors to plasma cells, T-cells, monocytes, NK cells and DCs (at protein level), and myeloma cells. Research studies have shown that BST2 also functions as an inhibitor of retrovirus release from human cells whose activity is antagonized by the HIV-1 accessory protein, Vpu. While BST2 causes retention of virions on cell surfaces or endocytosis into BST2-positive compartments, its depletion abolished the viral requirement for Vpu for virus release, an activity that may represent a potential therapeutic strategy for the treatment of HIV/AIDS. Another disease associated with BST2 dysfunction is stomatitis.仅用于科研。不用于诊断过程。
数据 |
| CD317 (BST2, PDCA-1) Antibody (16-3179-82)Cell host and microbe 2014 -Published figure using CD317 (BST2, PDCA-1) monoclonal antibody (Product # 16-3179-82) |
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参考文献： |
| 阻断1.Journal of immunology (Baltimore, Md. : 1950)Ig-like transcript 7, but not bone marrow stromal cell antigen 2 (also known as HM1.24, tetherin, or CD317), modulates plasmacytoid dendritic cell function in primary human blood leukocytes.2.Cell host and microbeNef proteins of epidemic HIV-1 group O strains antagonize human tetherin."Published figure using CD317 (BST2, PDCA-1) monoclonal antibody (Product # 16-3179-82) in Flow Cytometry" |
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