Mouse anti Human Estrogen Receptor Alpha
货号：MCA1799T,MCA1799
规格：0.1 ml,1ml
价格：1505,14306
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：Estrogen Receptor Alpha
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：6F11
荧光染料：Purified
| 产品介绍 | | |
| Mouse anti Human estrogen alpha antibody, clone 6F11recognizes the human estrogen receptor alpha chain (ERα), also known as the estradiol receptor or nuclear receptor subfamily 3 group A member 1. ERα is a ~65 kDa steroid hormone receptor containing an N-terminal (AF-1) ligand independent transactivation domain, a DNA binding domain and a C-terminal ligand binding domain which overlaps with an (AF-2) domain. ERα binds to DNA as a homodimer (Klinge 2001) and can also form heterodimers with Estrogen receptor beta.The detection of estrogen (ER) and progesterone (PR) receptors using immunohistochemical staining of formalin fixed, paraffin embedded (FFPE) tissue, has gradually replaced ligand binding assays, to become the most common method for the determination of the ER/PR status of breast tumors (Yazijiet al.2008). Approximately 75% to 80% of breast tumors have estrogen and/or progesterone receptors, and the presence of these receptors helps determine both the patients prognosis and the effectiveness of hormonal therapy (Bhargavaet al.2012).Mouse anti human estrogen alpha antibody, clone 6F11 has been used successfully for identification of ERα on breast cancer cell lysates using Western blotting and for the immunohistochemical detection of ERα in breast cancer tissues (Ambroiseet al.2011). |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Tissue Culture Supernatant - liquid |
| Species Cross-Reactivity | Target Species | Cross Reactivity |
| Human | √ |
| Dog | √ |
| nPreparatio | Recombinant human estrogen receptor (alpha form). |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | Pack Size: 1 mlPack Size: 0.1 ml<0.1% Sodium Azide (NaN3) |
| Approx. Protein Concentrations | IgG concentration 1.0 mg/ml |
| Fusion Partners | Spleen cells from immunised mice were fused with cells of the mouse p3-NS1-Ag4-1 myeloma cell line. |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Immunohistology - Frozen1 | √ | 1/40 | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
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| 文献 |
| 1.Bevitt, D.J.et al.(1997) New monoclonal antibodies to oestrogen and progesterone receptorective for paraffin section immunohistochemistry.J Pathol. 183 (2): 228-32.2.Jongen, V.et al.(2009) Expression of estrogen receptor-alpha and -beta and progesterone receptor-A and -B in a large cohort of patients with endometrioid endometrial cancer.Gynecol Oncol. 112: 537-42.3.Ambroise, M.et al.(2011) Immunohistochemical profile of breast cancer patients at a tertiary care hospital in South India.Asian Pac J Cancer Prev. 12: 625-9. |
技术参数 产品应用 WB
产品类型 Monoclonal Antibody
推荐用于 WB
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